Recombinant Human CD109 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 4385-CD
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human CD109 protein
Val22-Ser1268 (Tyr703Ser & Thr1241Met), with a C-terminal 10-His tag
Val22-Ser1268 (Tyr703Ser & Thr1241Met), with a C-terminal 10-His tag
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Val22
Predicted Molecular Mass
141 kDa
SDS-PAGE
155-165 kDa, reducing conditions
Activity
Measured by its ability to bind TGF-beta 1 in a functional ELISA. Finnson, K.W. et al. (2006) FASEB J. 20:1525.
Immobilized rhTGF-beta 1 at 1 µg/mL (100 µL/well) can bind rhCD109 with an apparent KD <0.1 µM.
Immobilized rhTGF-beta 1 at 1 µg/mL (100 µL/well) can bind rhCD109 with an apparent KD <0.1 µM.
Formulation, Preparation and Storage
4385-CD
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CD109
CD109 is a GPI-anchored member of the alpha-2-macroglobulin (A2M) and complement family of proteins (1). Mature human CD109 contains a bait region with recognition sequences for multiple proteases, an internal thioester bond, and a domain similar to the receptor binding domain of A2M (2). Cleavage of A2M family proteins within the bait region activates the thioester bond to promote covalent bonding to nucleophilic groups in adjacent molecules (3, 4). Within the region included in this recombinant protein, human CD109 shares71% - 73% amino acid (aa) sequence identity with mouse and rat CD109. It shares 27% - 33% aa sequence identity with A2M and complement factors C3, C4, and C5. Alternate splicing of human CD109 generates two isoforms with short deletions and one that is truncated within the bait region. CD109 is expressed on activated T cells and platelets, hematopoietic stem cells, megakaryocyte precursors, vascular endothelial cells, basal and myoepithelial cells of secretory glands, and squamous cell carcinomas (2, 5 - 9). It is produced as a 170 kDa - 180 kDa glycoprotein that is autocatalytically processed to 150 kDa and 120 kDa forms (2, 6, 10). CD109 on keratinocytes binds TGF-beta and associates with TGF-beta RI and TGF-beta RII, resulting in inhibition of TGF-beta signaling (11). Polymorphisms of CD109 include the platelet-specific Gov antigen and the blood group ABH antigens (12, 13). Alloantibodies directed against these antigens result in unsuccessful platelet transfusions, neonatal alloimmune thrombocytopenia, and posttransfusion purpura (14).
References
- Travis, J. and G.S. Salvesen (1983) Annu. Rev. Biochem. 52:655.
- Lin, M. et al. (2002) Blood 99:1683.
- Christensen, U. and L. Sottrup-Jensen (1984) Biochemistry 23:6619.
- Wallis, R. et al. (2007) J. Biol. Chem. 282:7844.
- Murray, L.J. et al. (1999) Exp. Hematol. 27:1282.
- Haregewoin, A. et al. (1994) Cell. Immunol. 156:357.
- Hasegawa, M. et al. (2007) Pathol. Int. 57:245.
- Brashem-Stein, C. et al. (1988) J. Immunol. 140:2330.
- Hashimoto, M. et al. (2004) Oncogene 23:3716.
- Solomon, K.R. et al. (2004) Gene 327:171.
- Finnson, K.W. et al. (2006) FASEB J. 20:1525.
- Schuh, A.C. et al. (2002) Blood 99:1692.
- Kelton, J.G. et al. (1998) J. Lab. Clin. Med. 132:142.
- Rozman, P. (2002) Transpl. Immunol. 10:165.
Alternate Names
CD109, CPAMD7
Gene Symbol
CD109
UniProt
Additional CD109 Products
Product Documents for Recombinant Human CD109 Protein, CF
Product Specific Notices for Recombinant Human CD109 Protein, CF
For research use only
Loading...
Loading...
Loading...