Recombinant Human CD117/c-kit His-tag Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI10931

Biotinylated
R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
AVI10931-050
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

P10721.1

Structure / Form

Biotinylated via Avi-tag.

Conjugate

Biotin

Applications

Bioactivity

View all CD117/c-kit Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CD117/c-kit protein
Human CD117/c-kit
(Gln26-Thr520)
Accession # P10721.1		 HHHHHH Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Protein identity confirmed by mass spectrometry.

Predicted Molecular Mass

58 kDa

SDS-PAGE

85-105 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human SCF (Catalog # 7644-SC) is coated at 1.00 μg/mL, 100 μL/well, Biotinylated Recombinant Human CD117/c-kit His-tag Avi-tag binds with an ED50 of 0.600-3.60 μg/mL.

Scientific Data Images for Recombinant Human CD117/c-kit His-tag Avi-tag Protein, CF

Biotinylated Recombinant Human CD117/c-kit His-tag Avi-tag Protein Binding Activity.

When Recombinant Human SCF (7644-SC) is coated at 1.00 ug/mL, 100 uL/well, Biotinylated Recombinant Human CD117/c-kit His-tag Avi-tag (Catalog # AVI10931) binds with an ED50 of 0.6-3.6 ug/mL.

Biotinylated Recombinant Human CD117/c-kit His-tag Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human CD117/c-kit His-tag Avi-tag Protein (Catalog # AVI10931) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 85-105 kDa.

Formulation, Preparation and Storage

AVI10931
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD117/c-kit

Stem cell factor receptor (SCFR), also known as c-Kit and CD117, is a type III receptor tyrosine kinase that acts as the receptor for the cytokine SCF (1, 2). Mature human SCFR consists of an extracellular domain (ECD) with five tandem immunoglobulin-like domains, a single transmembrane segment, and a cytoplasmic region with a split tyrosine kinase domain. Within the ECD, human SCFR shares 73% and 76% amino acid sequence identity with mouse and rat SCFR, respectively. At least 4 SCFR isoforms, produced by alternative mRNA splicing, have been identified in humans and 2 are characterized by the presence or absence of a tetrapeptide sequence (GNNK) in the juxtamembrane region of the ECD (3, 4). Human SCFR is expressed in embryonic stem cells and is involved in regulation of cell survival and proliferation, hematopoiesis, gametogenesis, and melanogenesis (1, 2, 5, 6). SCF R interacts with noncovalent dimers of transmembrane or soluble SCF to trigger receptor dimerization and initiate signaling (7). Our Avi-tag Biotinylated Recombinant Human SCFR features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Roskoski, R. et al. (2005) Bioche. & Biophy Res Comm. 338:1307.
  2. Ashman, L.K. et al. (1999) Inter Jour of Biochem & Cell Bio. 31:1037.
  3. Lennartsson, J. and Rönnstrand L. (2012) Physiol Rev. 92:1619.
  4. Caruana, G. et al. (1999) Oncogene 18:5573.
  5. Wang, C.-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540.
  6. Erlandsson, A. et al. (2004) Exp. Cell Res. 301:201.
  7. Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.

Long Name

Stem Cell Factor Receptor

Alternate Names

c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR

Entrez Gene IDs

3815 (Human); 16590 (Mouse); 64030 (Rat)

Gene Symbol

KIT

UniProt

P10721.1

Additional CD117/c-kit Products

Product Documents for Recombinant Human CD117/c-kit His-tag Avi-tag Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human CD117/c-kit His-tag Avi-tag Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.