Recombinant Human CD157 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 4736-AC
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human CD157 protein
Gly29-Lys292, with a C-terminal 6-His tag
Gly29-Lys292, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Gly29
Predicted Molecular Mass
31 kDa
SDS-PAGE
38 kDa and 40 kDa, reducing conditions
Activity
Measured by its ability to convert the substrate nicotinamide guanine dinucleotide (NGD+) to cyclic GDP-ribose. Graeff, R.M. et al. (1994) J. Biol. Chem. 269:30260.
The specific activity is >20 pmol/min/µg, as measured under the described conditions.
The specific activity is >20 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
4736-AC
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CD157
CD157, also known as bone marrow stromal cell antigen 1 (BST-1), is a glycosyl phosphatidylinositol anchored membrane protein that belongs to the CD38 family (1). CD157 was discovered in a bone marrow stromal cell line where it facilitates pre-B-cell growth (2, 3). Along with CD38, CD157 is a bifunctional ectoenzyme that exhibits both ADP-ribosyl cyclase and cyclic ADP ribose hydrolase activities (2). It may play a role in rheumatoid arthritis (RA) due to its enhanced expression in RA‑derived bone marrow stromal cell lines (3). CD157 has been predicted to function as a cell surface receptor and an immunoregulatory molecule (4).
References
- Hussain, A. M. M. et al. (1998) Protein Express. Purif. 12:133.
- Sato, A. et al. (1999) Biochem. J. 337:491.
- Kaisho, T. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5325.
- Ortolan, E. et al. (2002) Cell Biochem. Funct. 20:309.
Long Name
Cluster of Differentiation 157
Alternate Names
BP-3, BST1, cADPr hydrolase 2, CD157
Gene Symbol
BST1
UniProt
Additional CD157 Products
Product Documents for Recombinant Human CD157 Protein, CF
Product Specific Notices for Recombinant Human CD157 Protein, CF
For research use only
Loading...
Loading...
Loading...