Recombinant Human CD24 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5247-CD

R&D Systems, part of Bio-Techne
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5247-CD-050
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Key Product Details

Source

NS0

Accession #

NP_037362

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all CD24 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CD24 protein
Human CD24
(Met1 - Gly59)
Accession # NP_037362		 IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser27

Predicted Molecular Mass

29.7 kDa (monomer)

SDS-PAGE

45-55 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.

When Recombinant Human CD24 Fc Chimera is coated at 5 μg/mL, the concentration of Recombinant Human Siglec-10 Fc Chimera (Catalog # 2130-SL) that produces 50% optimal binding response is found to be approximately 0.3-3 μg/mL.

Reviewed Applications

Read 2 reviews rated 5 using 5247-CD in the following applications:

Formulation, Preparation and Storage

5247-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD24

CD24, also known as Heat-Stable Antigen and Nectadrin, is a heavily and variably glycosylated 30 kDa ‑ 60 kDa GPI-linked sialoprotein (1 - 4). Human CD24 is expressed on B lineage cells and granulocytes, on epithelial, neuronal, and muscle cells, and on a range of tumor cells (4 - 6). In mouse, CD24 is even more widely expressed, particularly on T cells, monocytes, and dendritic cells (2). CD24 expression is regulated during lineage development and with the activation of various cell types (4). Antibody crosslinking of CD24 enhances the induction of apoptosis in B and T lymphocytes which contributes to negative selection and the induction of immune tolerance (7 - 11). CD24 on antigen presenting cells cooperates with B7 molecules in the costimulation of T cells (12 - 14). CD24 associates in cis with Siglec-10 (or Siglec-G in mouse) and with the danger-associated molecules HMGB1, HSP70, or HSP90 which are released from necrotic or damaged cells (15). Formation of these ternary complexes fills a protective role: the resulting Siglec-10 signaling inhibits inflammatory responses that are otherwise induced by extracellular DAMPs (15, 16). Mature human CD24 shares 30% and 42% amino acid sequence identity with mouse and rat CD24, respectively.

References

  1. Jackson, D. et al. (1992) Cancer Res. 52:5264.
  2. Kay, R. et al. (1991) J. Immunol. 147:1412.
  3. Kadmon, G. et al. (1992) J. Cell Biol. 118:1245.
  4. Fang, X. et al. (2010) Cell. Mol. Immunol. 7:100.
  5. Fischer, G. F. et al. (1990) J. Immunol. 144:638.
  6. Allman, D.M. et al. (1993) J. Immunol. 151:4431.
  7. Suzuki, T. et al. (2001) J. Immunol. 166:5567.
  8. Taguchi, T. et al. (2003) J. Immunol. 170:252.
  9. Jung, K.C. et al. (2004) J. Immunol. 172:795.
  10. Kishimoto, H. and J. Sprent (1997) J. Exp. Med. 185:263.
  11. Hitsumoto, Y. et al. (1996) Immunology 89:200.
  12. Liu, Y. et al. (1992) Eur. J. Immunol. 22:2855.
  13. Enk, A.H. and S.I. Katz (1994) J. Immunol. 152:3264.
  14. Askew, D. and C.V. Harding (2008) Immunology 123:447.
  15. Chen G.Y. et al. (2009) Science 323:1722.
  16. Liu, Y. et al. (2011) Curr. Opin. Immunol. 23:41.

Alternate Names

CD24, CD24A

Entrez Gene IDs

100133941 (Human); 12484 (Mouse)

Gene Symbol

CD24

UniProt

NP_037362

Additional CD24 Products

Product Documents for Recombinant Human CD24 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human CD24 Fc Chimera Protein, CF

For research use only

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