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Recombinant Human CD3 delta/CD3d Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10280-TC

R&D Systems, part of Bio-Techne
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10280-TC-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CD3 delta/CD3d protein
Human CD3 delta/CD3d
(Phe22-Ala105)
Accession # P04234
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe22

Predicted Molecular Mass

36 kDa

SDS-PAGE

45-55 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CD3 delta/CD3d Fc Chimera (Catalog # 10280-TC) is immobilized at 1.0 μg/mL, 100 μL/well, the concentration of Recombinant Human CD3E Fc Chimera Biotinylated Protein that produces 50% of the optimal binding response is approximately 1.0-6.0 μg/mL.

Scientific Data Images for Recombinant Human CD3 delta/CD3d Fc Chimera Protein, CF

Recombinant Human CD3 delta/CD3d Fc Chimera Protein Binding Activity

Recombinant Human CD3 delta/CD3d Fc Chimera Protein Binding Activity

When Recombinant Human CD3 delta/CD3d Fc Chimera (Catalog # 10280-TC) is immobilized at 1.0 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human CD3E Fc Chimera that produces 50% of the optimal binding response is approximately 1.0-6.0 μg/mL.
Recombinant Human CD3 delta/CD3d Fc Chimera Protein SDS-PAGE

Recombinant Human CD3 delta/CD3d Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human CD3 delta/CD3d Fc Chimera (Catalog # 10280-TC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 45-55 kDa and 90-110 kDa, respectively.

Formulation, Preparation and Storage

10280-TC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD3 delta/CD3d

CD3d, also known as CD3 delta and T3D, is a single-pass type I membrane protein. CD3 delta/CD3d is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction (1). The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T cells (2). CD3 delta/CD3d contains an 84 amino acid extracellular domain, a 21 amino acid transmembrane domain, and a 45 amino acid cytoplasmic domain. Human CD3 delta/CD3d shares 89%, 61%, and 57% sequence identity in its extracellular domain with its cynomolgus monkey, rat, and mouse homologs, respectively. CD3 delta/CD3d, like other subunit members CD3G and CD3E of the TCR-CD3 complex, contains an extracellular Ig domain and a single immunoreceptor tyrosine-based activation motif (3, 4). Defects in CD3 delta/CD3d cause severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T-/B+/NK+ SCID) which is a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels (5, 6).

References

  1. Gil, J. et al. (2011) J. Clin. Invest. 121:3872.
  2. Carson, G.R. et al. (1991) J. Biol. Chem. 266:7883.
  3. Birnbaum, M.E. et al. (2014). Proc. Natl. Acad. Sci. USA. 111:17576.
  4. Wucherpfennig, K. et al. (2010). Cold Spring Harb Perspect. Biol. 2:a005140.
  5. Kim, S.T. et al. (2010) J. Immunol. 185:2951.
  6. Holst, J. et al. (2008) Nat. Immunol. 9:658.

Long Name

CD3 delta

Alternate Names

CD3D

Entrez Gene IDs

915 (Human); 12500 (Mouse); 25710 (Rat); 102133701 (Cynomolgus Monkey)

Gene Symbol

CD3D

UniProt

Additional CD3 delta/CD3d Products

Product Documents for Recombinant Human CD3 delta/CD3d Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human CD3 delta/CD3d Fc Chimera Protein, CF

For research use only

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