Recombinant Human CD3 epsilon Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9850-CD

Analyzed by SEC-MALS.
R&D Systems, part of Bio-Techne
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9850-CD-050
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Key Product Details

Source

HEK293

Accession #

P07766

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all CD3 epsilon Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CD3 epsilon protein
Human CD3 epsilon
(Gln22-Asp126)
Accession # P07766		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln22 inferred from enzymatic pyroglutamate treatment revealing Asp23.

Predicted Molecular Mass

38 kDa

SDS-PAGE

40-57 kDa, reducing conditions

Activity

Bioassay data are not available.

Scientific Data Images for Recombinant Human CD3 epsilon Fc Chimera Protein, CF

Recombinant Human CD3 epsilon Fc Chimera Protein SEC-MALS.

Recombinant Human CD3 epsilon Fc Chimera (Catalog # 9850-CD) has a molecular weight (MW) of 81.3 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

Formulation, Preparation and Storage

9850-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: CD3 epsilon

CD3 epsilon (T-Cell Surface Glycoprotein CD3 Epsilon) is a type I transmembrane protein that belongs to the Ig (Immunoglobulin) superfamily. Human CD3 epsilon consists of 104 amino acid (aa) extracellular domain, 26 aa transmembrane domain and 55 aa cytoplasmic domain (1). With the extracellular domain, human CD3 epsilon shares 44.2% and 49% homology with mouse and rat CD3 epsilon respectively. There are four CD3 proteins: CD3 delta, CD3 epsilon, CD3 gamma, and CD3 zeta. All CD3 proteins contain ITAM (Immunoreceptor Tyrosine-based Activation Motifs) in the cytoplasmic tail, which becomes phosphorylated by Src family protein tyrosine kinases LCK and FYN upon TCR engagement (2). CD3 proteins form heterodimers of CD3 delta/CD3 epsilon and CD3 gamma/CD3 epsilon, which bind to TCR and form trimeric TCR alpha/CD3 epsilon/CD3 gamma and TCR beta/CD3 gamma/CD3 epsilon. The resulting heterohexamer further associates with CD3 zeta homodimer and forms TCR/CD3 signaling complex (3, 4). Similar complex is also formed with TCR gamma and TCR delta (5). CD3 epsilon plays essential role in adaptive immune response. Mutations in CD3 epsilon lead to SCID (severe combined immunodeficiency) (6).

References

  1. Gold, D.P. et al. (1986) Nature 321:431.
  2. Barber, E.K. et al. (1989) Proc. Nat. Acad. Sci. U.S.A. 86:3277.
  3. Manolios, N. et al. (1991) EMBO J 10:1643.
  4. Dietrich, J. et al. (1999) Eur J Immunol 29:1719.
  5. Pan, Q. et al. (2006) Mol Immunol 43:1741.
  6. Soudais, C. et al. (1993) Nat. Genet. 3:77.

Alternate Names

CD3e

Entrez Gene IDs

916 (Human); 12501 (Mouse); 102133065 (Cynomolgus Monkey)

Gene Symbol

CD3E

UniProt

P07766

Additional CD3 epsilon Products

Product Documents for Recombinant Human CD3 epsilon Fc Chimera Protein, CF

Product Datasheets

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