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Recombinant Human CD30 Ligand/TNFSF8 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 1028-CL

R&D Systems, part of Bio-Techne
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1028-CL-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CD30 Ligand/TNFSF8 protein
MHHHHHHHHHH GGGSGGGSGGGS IEGR Human CD30 Ligand
(Gln63-Asp234)
Accession # P32971
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

22 kDa

SDS-PAGE

30-40 kDa, reducing conditions

Activity

Measured by its ability to stimulate IL-6 secretion by HDLM human Hodgkin’s lymphoma cells. Duckett, C.S. et al. (1997) Mol. Cell. Biol. 17:1535.
The ED50 for this effect is 8-48 ng/mL in the presence of 10 µg/mL of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).

Formulation, Preparation and Storage

1028-CL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD30 Ligand/TNFSF8

CD30 ligand (CD30L)/TNFSF8 is a type II membrane protein belonging to the TNF superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. The protein is also constitutively expressed on granulocytes and medullary thymic epithelial cells. The specific receptor for CD30L is CD30/TNFRSF8, a type I transmembrane glycoprotein belonging to the TNF receptor superfamily. CD30 was originally identified as a cell surface antigen of Hodgkin's and Reed-Sternberg cells using the monoclonal antibody Ki-1. CD30 is also expressed on different non-Hodgkin's lymphomas, virus-infected T and B cells, and on normal T and B cells after activation. Among T cells, CD30 is preferentially expressed on a subset of T cells producing Th2-type cytokines and on CD4+/CD8+ thymocytes that co-express CD45RO and IL-4 receptor. CD30 ligation by CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation and cell death by apoptosis. CD30 can act as a co-stimulatory molecule in thymic negative selection and may also play a critical role in the pathophysiology of Hodgkin's disease and other CD30+ lymphomas. Human and mouse CD30 ligand cDNAs share 70% sequence homology.

References

  1. Brunangelo, F. et al. (1995) Blood 85:1.
  2. Duckett, C.S. et al. (1997) Mol. Cell. Biol. 17:1535.
  3. Chiarle, R. et al. (1999) J. Immunol. 163:194.

Alternate Names

CD153, CD30L, TNFSF8

Entrez Gene IDs

944 (Human); 21949 (Mouse)

Gene Symbol

TNFSF8

UniProt

Additional CD30 Ligand/TNFSF8 Products

Product Documents for Recombinant Human CD30 Ligand/TNFSF8 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human CD30 Ligand/TNFSF8 Protein, CF

For research use only

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