Recombinant Human CD31/PECAM-1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # ADP6
Key Product Details
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human CD31/PECAM-1 protein
Gln28-Lys601
Gln28-Lys601
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
No results obtained: Gln28 predicted
Predicted Molecular Mass
64.5 kDa
Activity
Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells.
When 5 x 104 cells/well are added to rhCD31 coated plates (3.3 μg/mL, 100 μL/well) in the presence of 20 ng.mL PMA, approximately 50-65% will adhere after 30 minutes at 37°C.
When 5 x 104 cells/well are added to rhCD31 coated plates (3.3 μg/mL, 100 μL/well) in the presence of 20 ng.mL PMA, approximately 50-65% will adhere after 30 minutes at 37°C.
Formulation, Preparation and Storage
ADP6
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS containing Calcium and Magnesium with Sorbitol. |
| Reconstitution |
Reconstitute at 150 μg/mL in sterile, deionized water.
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| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: CD31/PECAM-1
The CD31 adhesion molecule, also known as PECAM-1, is expressed in large amounts on endothelial cells at intercellular junctions and on T cell subsets, and to a lesser extent on platelets and most other leukocytes such as monocytes and neutrophils. CD31 binds to itself homotypically, and also to the leukocyte integrin alphav beta3 heterotypically. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells. CD31 has been found in human plasma, and the presence of this circulating isoform is suggested to modulate the transendothelial migration of leukocytes.
Long Name
Platelet Endothelial Cell Adhesion Molecule 1
Alternate Names
CD31, EndoCAM, PECA1, PECAM-1, PECAM1
Gene Symbol
PECAM1
UniProt
Additional CD31/PECAM-1 Products
Product Documents for Recombinant Human CD31/PECAM-1 Protein, CF
Product Specific Notices for Recombinant Human CD31/PECAM-1 Protein, CF
For research use only
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