Recombinant Human CD320/TCblR/8D6A Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1557-CD
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human CD320/TCblR/8D6A protein
| Human CD320 (Leu31-Val231) Accession # Q9NPF0 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus |
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Leu31 and Ser36
Predicted Molecular Mass
47.2 kDa (monomer)
SDS-PAGE
80-90 kDa, reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
When Recombinant Human CD320/TCblR/8D6A Fc Chimera is immobilized at 25 ng/mL (100 μL/well), the concentration of Recombinant Human Transcobalamin II (Catalog # 7895-TC) that produces 50% of the optimal binding response is 0.5-3 ng/mL.
When Recombinant Human CD320/TCblR/8D6A Fc Chimera is immobilized at 25 ng/mL (100 μL/well), the concentration of Recombinant Human Transcobalamin II (Catalog # 7895-TC) that produces 50% of the optimal binding response is 0.5-3 ng/mL.
Scientific Data Images for Recombinant Human CD320/TCblR/8D6A Fc Chimera Protein, CF
Recombinant Human CD320/TCblR/8D6A Fc Chimera Protein Bioactivity
When Recombinant Human CD320/TCblR/8D6A Fc Chimera (Catalog # 1557-CD) is coated at 25 ng/mL, Recombinant Human Transcobalamin II (Catalog # 7895-TC) binds with an ED50 of 0.5-3 ng/mL.Formulation, Preparation and Storage
1557-CD
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl. |
| Reconstitution |
Reconstitute at 200 μg/mL in PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: CD320/TCblR/8D6A
CD320, also known as the Transcobalamin II receptor (TCb1R) and 8D6A, is an approximately 60 kDa transmembrane member of the LDL receptor family (1). Mature human CD320 consists of a 199 amino acid (aa) extracellular domain with two cysteine-rich LDLR-A repeats, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain (2). Within the extracellular domain, human CD320 shares 52% aa sequence identity with mouse and rat CD320. CD320 is a heavily glycosylated protein that is expressed as a noncovalent dimer in the kidney, placenta, liver, intestine, and secondary lymphoid organs (3 - 5). It functions as an endocytic receptor for circulating complexes of Cobalamin (Vitamin B12) and Transcobalamin II (3). In the immune system, CD320 is expressed by germinal center B cells and follicular dendritic cells (FDC) and provides a co‑stimulatory signal to B cells during the germinal center reaction (2, 6 - 8). CD320 also cooperates with CD44 in promoting the development of B cell lymphomas (9).
References
- May, P. et al. (2007) Ann. Med. 39:219.
- Li, L. et al. (2000) J. Exp. Med. 191:1077.
- Quadros, E.V. et al. (2009) Blood 113:186.
- Bose, S. et al. (1995) J. Biol. Chem. 270:8152.
- Park, H.J. et al. (2009) Immune Netw. 9:138.
- Cho, W. et al. (2008) BMC Rep. 41:863.
- Zhang, X. et al. (2001) J. Immunol. 167:49.
- Park, C.-S. and Y.S. Choi (2005) Immunology 114:2.
- Li, L. et al. (2004) Blood 104:815.
Long Name
Transcobalamin II Receptor
Alternate Names
8D6A, CD320, TCblR
Gene Symbol
CD320
UniProt
Additional CD320/TCblR/8D6A Products
Product Documents for Recombinant Human CD320/TCblR/8D6A Fc Chimera Protein, CF
Product Specific Notices for Recombinant Human CD320/TCblR/8D6A Fc Chimera Protein, CF
For research use only
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