Recombinant Human CD35 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5748-CD

R&D Systems, part of Bio-Techne
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5748-CD-050
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Key Product Details

Source

NS0

Accession #

CAA68755

Conjugate

Unconjugated

Applications

Binding Activity

View all CD35 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CD35 protein
Gln42-Asp1971, with a C-terminal 10-His tag and a substitution of His1208Arg

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln42 predicted

Predicted Molecular Mass

213 kDa

SDS-PAGE

200-220 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When rhCD35 is coated at 5 μg/mL, complement component C3b binds with an apparent KD <10 nM.

Formulation, Preparation and Storage

5748-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 400 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD35

CD35, also known as complement receptor 1 (CR1), is an N-glycosylated member of the RCA (regulators of complement activation) family of proteins. The complement cascade plays an important role in the innate immune system through the recognition and clearance of immune complexes and foreign particles. There are four major allotypes of human CD35 which range from approximately 220 - 300 kDa (1, 2). Mature human CD35 contains a 1930 amino acid (aa) extracellular domain (ECD) with 30 tandem SCR/SUSHI repeats, a 25 aa transmembrane segment, and a 43 aa cytoplasmic tail (3). A mouse ortholog of human CD35 has not been described, although alternate splicing of mouse CD21/CR2 generates a protein with homology to some SCR repeats of human CD35 (4). A soluble form of the CD35 ECD circulates in the serum (5). Cell surface CD35 is widely expressed on hematopoietic cells (1). It cooperates with both CD21 and CR3/MAC-1 in the binding and internalization of particles and immune complexes that are opsonized with complement components C3b, C3i, and C4b (1, 6, 7). It also binds and internalizes particles opsonized with complement factor C1q or mannose binding lectin (MBL) (8, 9). CD35 protects the cell from complement-mediated lysis by serving as a cofactor for Factor I and inhibiting the C3 and C5 convertases (10). Erythrocyte-expressed CD35 mediates the hepatic clearance of complement-opsonized immune complexes (11). On lymphocytes, CD35 inhibits the activation-induced proliferation of T cells and B cells (12, 13). Mice deficient in both CD21 and CD35 exhibit normal B cell development but severely compromised germinal center development, antibody production, and establishment of protective microbial immunity (14). In mice, CD21/CD35 must additionally be present on follicular dendritic cells to mount effective humoral responses and establishment of B cell memory (15).

References

  1. Roozendaal, R. and M.C. Carroll (2007) Immunol. Rev. 219:157.
  2. Wong, W.W. et al. (1989) J. Exp. Med. 169:847.
  3. Klickstein, L.B. et al. (1987) J. Exp. Med. 165:1095.
  4. Kurtz, C.B. et al. (1990) J. Immunol. 144:3581.
  5. Yoon, S.H. and D.T. Fearon (1985) J. Immunol. 134:3332.
  6. Tuveson, D.A. et al. (1991) J. Exp. Med. 173:1083.
  7. Sutterwala, F.S. et al. (1996) J. Leukoc. Biol. 59:883.
  8. Klickstein, L.B. et al. (1997) Immunity 7:345.
  9. Ghiran, I. et al. (2000) J. Exp. Med. 192:1797.
  10. Iida, K. and V. Nussenzweig (1981) J. Exp. Med. 153:1138.
  11. Cornacoff, J.B. et al. (1983) J. Clin. Invest. 71:236.
  12. Wagner, C. et al. (2006) Mol. Immunol. 43:643.
  13. Jozsi, M. et al. (2002) J. Immunol. 168:2782.
  14. Haas, K.M. et al. (2002) Immunity 17:713.
  15. Rossbacher, J. et al. (2006) Eur. J. Immunol. 36:2384.

Alternate Names

C3BR, CD35, CR1, KN

Entrez Gene IDs

1378 (Human)

Gene Symbol

CR1

UniProt

CAA68755

Additional CD35 Products

Product Documents for Recombinant Human CD35 Protein, CF

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