Recombinant Human CD38 His-tag Biotin Protein, CF

Name: Recombinant Human CD38 His-tag Biotin Protein, CF
Brand: R&D Systems
SKU: BT2404

R&D Systems, part of Bio-Techne | Catalog # BT2404

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Product Datasheet / COA / SDS

Key Product Details

Source:	NS0
Accession #:	P28907.2
Structure / Form:	Biotinylated via amines
Applications:	Bioactivity, Enzyme Activity

View Full Technical Details for Recombinant Human CD38 His-tag Biotin Protein, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Human CD38 His-tag Biotin Protein, CF

Source	Mouse myeloma cell line, NS0-derived human CD38 protein Val43-Ile300 with a C-terminal 6-His tag
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Val43
Predicted Molecular Mass	31 kDa
SDS-PAGE	31 kDa
Activity	Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human CD38 His-tag binds to Human CD38 Antibody (Catalog # AF2404) with an ED₅₀of 1.50-15.0 ng/mL. Measured by its ability to convert the substrate nicotinamide guanine dinucleotide (NGD⁺) to cyclic GDP-ribose. Graeff, R.M. et al. (1994) J. Biol. Chem. 269:30260. The specific activity is >2200 pmol/min/μg, as measured under the described conditions.

Scientific Data Examples for Recombinant Human CD38 His-tag Biotin Protein, CF

	Biotinylated Recombinant Human CD38 His-tag Binding Activity. Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human CD38 His-tag binds to Human CD38 Antibody (AF2404) with an ED₅₀ of 1.50‑15.0 ng/mL.
	Biotinylated Recombinant Human CD38 His-tag Protein Enzyme Activity. Biotinylated Recombinant Human CD-38 His-tag Protein (Catalog # BT2404) is measured by its ability to convert the substrate nicotinamide guanine dinucleotide (NGD+) to cyclic GDP-ribose.
	Biotinylated Recombinant Human CD38 His-tag Protein SDS-PAGE. 2 μg/lane of Biotinylated Recombinant Human CD38 His-tag Protein (Catalog # BT2404) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-44 kDa, under reducing conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT2404

Formulation	Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD38

CD38, also known as ADP-ribosyl cyclase, converts NAD(P)+ into three separate products with calcium mobilizing ability: cyclic ADP-ribose, NAADP+, and ADP-ribose (1). CD38 is a Type II transmembrane glycoprotein composed of an intracellular domain, a single transmembrane helix domain, and a large extracellular domain that contains the catalytic site (2). CD38 is expressed in B and T lymphocytes, osteoclasts, and in cardiac, pancreatic, liver and kidney cells (3, 4). Through its production of cyclic ADP-ribose, CD38 modulates calcium-mediated signal transduction in many types of cells (5, 6). CD38 is also reported to bind as a receptor to trigger signaling cascades (7, 8). Through both mechanisms, CD38 influences proliferation and trafficking (8, 9). CD38 is used as a marker for poor prognosis in chronic lymphocytic leukemia and multiple myeloma and is an attractive cancer immunotherapy drug target (8-11).

Item	Value
References	Schuber, F. and F.E. Lund (2004) Curr. Mol. Med. 4:249. Liu, Q. et al. (2005) Structure. 13:1331. Jackson, D.G. and J.I. Bell (1990) J. Immunol. 144:2811. Sun, L. et al. (1999) J. Cell Biol. 146:1161. Partida-Sanchez, S. et al. (2001) Nature Med. 7:1209. Kato, I. et al. (1995) J. Biol. Chem. 270:30045. Deaglio, S. (2000) Chem. Immunol. 75:99. Chillemi,A. et al. (2013) Mol. Med. 19:99. Vaisitti, T. et al. (2015) Leukemia. 29:356. Atanackovic, D. et al. (2016) Oncoimmunology. 5:e1217374. Tai, Y-T. et al. (2017) Oncotarget. 8:112166.
Long Name	Cluster of Differentiation 38
Entrez Gene IDs	952 (Human); 12494 (Mouse); 25668 (Rat); 102126394 (Cynomolgus Monkey)
Alternate Names	2'-phospho-ADP-ribosyl cyclase, 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase (EC:2.4.99.20), 2'-phospho-cyclic-ADP-ribose transferase, ADP-ribosyl Cyclase, ADP-ribosyl cyclase 1, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, ADPRC 1, ADPRC1, CD38, CD38 antigen, CD38 antigen (p45), CD38 molecule, CD_antigen: CD38, Cyclic ADP-ribose Hydrolase, Cyclic ADP-ribose hydrolase 1, EC 2.4.99.20, EC 3.2.2.6, EC:3.2.2.6, NAD(+) nucleosidase, T10, cADPr hydrolase 1, cluster of differentiation 38

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Recombinant Human CD38 His-tag Biotin Protein, CF

R&D Systems, part of Bio-Techne | Catalog # BT2404

Key Product Details

Product Specifications for Recombinant Human CD38 His-tag Biotin Protein, CF

Scientific Data Examples for Recombinant Human CD38 His-tag Biotin Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: CD38

Customer Reviews for Recombinant Human CD38 His-tag Biotin Protein, CF

Product Documents for Recombinant Human CD38 His-tag Biotin Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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