Recombinant Human CD38 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2404-AC
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human CD38 protein
Val43-Ile300, with a C-terminal 6-His tag
Val43-Ile300, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Val43
Predicted Molecular Mass
31 kDa
SDS-PAGE
43 kDa, reducing conditions
Activity
Measured by its ability to convert the substrate nicotinamide guanine dinucleotide (NGD+) to cyclic GDP-ribose. Graeff, R.M. et al. (1994) J. Biol. Chem. 269:30260.
The specific activity is >2,500 pmol/min/µg, as measured under the described conditions.
The specific activity is >2,500 pmol/min/µg, as measured under the described conditions.
Scientific Data Images for Recombinant Human CD38 Protein, CF
Recombinant Human CD38 Protein Enzyme Activity.
Recombinant Human CD38 Protein (Catalog # 2404-AC) is measured by its ability to convert the substrate nicotinamide guanine dinucleotide (NGD+) to cyclic GDP-ribose.Formulation, Preparation and Storage
2404-AC
| Formulation | Supplied as a 0.2 μm filtered solution in MES and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CD38
CD38, also known as ADP-ribosyl cyclase, is a Type II integral membrane protein. The enzyme is able to transform NAD(P)+ into three different products with calcium mobilizing ability, cyclic ADP-ribose, NAADP+, and ADP-ribose (1). CD38 is expressed in B and T lymphocytes, osteoclasts, and in cardiac, pancreatic, liver and kidney cells (2, 3). Through its production of cyclic ADP-ribose, CD38 modulates calcium-mediated signal transduction in many types of cells, including neutrophils and pancreatic beta cells (4, 5).
References
- Schuber, F. and F.E. Lund (2004) Curr. Mol. Med. 4:249.
- Jackson, D.G. and J.I. Bell (1990) J. Immunol. 144:2811.
- Sun, L. et al. (1999) J. Cell Biol. 146:1161.
- Partida-Sanchez, S. et al. (2001) Nature Med. 7:1209.
- Kato, I. et al. (1995) J. Biol. Chem. 270:30045.
Long Name
Cluster of Differentiation 38
Alternate Names
ADP-ribosyl Cyclase, CD38, Cyclic ADP-ribose Hydrolase
Gene Symbol
CD38
UniProt
Additional CD38 Products
Product Documents for Recombinant Human CD38 Protein, CF
Product Specific Notices for Recombinant Human CD38 Protein, CF
For research use only
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