Recombinant Human CD4 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 10327-CD
Key Product Details
Source
CHO
Accession #
Structure / Form
Monomer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human CD4 protein
Lys26-Trp390
Lys26-Trp390
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Lys26
Predicted Molecular Mass
41 kDa
SDS-PAGE
48-52 kDa, under reducing conditions
Activity
Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells.
The ED50 for this effect is 0.75-4.5 μg/mL.
The ED50 for this effect is 0.75-4.5 μg/mL.
Reviewed Applications
Read 2 reviews rated 4 using 10327-CD in the following applications:
Scientific Data Images for Recombinant Human CD4 Protein, CF
Recombinant Human CD4 Protein Bioactivity
Immobilized Recombinant Human CD4 Protein (Catalog # 10327-CD) supports the adhesion of HeLa human cervical epithelial carcinoma cells. The ED50 for this effect is 0.75-4.5 μg/mL.Recombinant Human CD4 Protein SDS-PAGE
2 μg/lane of Recombinant Human CD4 (Catalog # 10327-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 48-52 kDa.Formulation, Preparation and Storage
10327-CD
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute at 200 μg/mL in PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CD4
References
- Vignali, D.A.A. (2010) J. Immunol. 184:5933.
- Maddon, P.J. et al. (1985) Cell 42:93.
- Alarcon, B. and H.M. van Santen (2010) Sci. Signal. 3:pe11.
- Wan, Y.Y. and R.A. Flavell (2009) Mol. Cell Biol. 1:20.
- Kitchen, S.G. et al. (2005) Proc. Natl. Acad. Sci. 102:3794.
- Crocker, P.R. et al. (1987) J. Exp. Med. 166:613.
- Biswas, P. et al. (2003) Blood 101:4452.
- Bernstein, H.B. et al. (2006) J. Immunol. 177:3669.
- Funke, I. et al. (1987) J. Exp. Med. 165:1230.
- Doyle, C. and J.L. Strominger (1987) Nature 330:256.
- Huppa, J.B. et al. (2010) Nature 463:963.
- Fragoso, R. et al. (2003) J. Immunol. 170:913.
- Cruikshank, W.W. et al. (1994) Proc. Natl. Acad. Sci. 91:5109.
- Klatzmann, D. et al. (1984) Nature 312:767.
- Dagleish, A.G. et al. (1984) Nature 312:763.
Alternate Names
CD4
Entrez Gene IDs
Gene Symbol
CD4
UniProt
Additional CD4 Products
Product Documents for Recombinant Human CD4 Protein, CF
Product Specific Notices for Recombinant Human CD4 Protein, CF
For research use only
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