Recombinant Human CD44v6 Fc Chimera Avi-tag Protein, CF

Name: Recombinant Human CD44v6 Fc Chimera Avi-tag Protein, CF
Brand: R&D Systems
SKU: AVI11175

R&D Systems, part of Bio-Techne | Catalog # AVI11175

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Product Datasheet / COA / SDS

Key Product Details

Structure / Form:	Disulfide-linked Homodimer Biotinylated via Avitag
Applications:	Bioactivity

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Technical Data
Citations / Reviews

Product Specifications for Recombinant Human CD44v6 Fc Chimera Avi-tag Protein, CF

N-terminal sequence Analysis	Gln21, inferred from enzymatic pyroglutamate treatment revealing Ile22
Predicted Molecular Mass	60 kDa
SDS-PAGE	60 kDa

Scientific Data Examples for Recombinant Human CD44v6 Fc Chimera Avi-tag Protein, CF

	Biotinylated Recombinant Human CD44v6 Fc Chimera Avi-tag Protein Binding Activity. Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human CD44v6 Fc Chimera Avi-tag Protein (Catalog # AVI11175) binds to Recombinant Human LSECtin/CLEC4G Protein (2947-CL) with an ED₅₀ of 40.0-400 ng/mL.
	Biotinylated Recombinant Human CD44v6 Fc Chimera Avi-tag Protein SDS-PAGE. 2 μg/lane of Biotinylated Recombinant Human CD44v6 Fc Chimera Avi-tag Protein (Catalog # AVI11175) was resolved with SDS-PAGE under reducing (R) condition and visualized by Coomassie® Blue staining, showing bands at 105-120 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI11175

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Stability & Storage:	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD44

CD44 is a ubiquitously expressed protein that is the major receptor for hyaluronan and exerts control over cell growth and migration (1-3). Human CD44 has a 20 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan-binding disulfide-stabilized link region and a 325-530 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. CD44 transcripts undergo complex alternative splicing, and, within the stem, ten variably spliced exons (v1‑10 corresponding to exons 6-15; although human CD44 lacks v1/exon 6) produce multiple protein isoforms (1-4). The standard or hematopoietic form, CD44H, does not include the variable segments (1-4). Cancer aggressiveness and T cell activation have been correlated with expression of specific isoforms (1, 4, 5). Human CD44v6 contains exon 11 (v6) and is involved in many biological processes including cell growth, apoptosis, and metastasis (6-7). With variable N- and O‑glycosylation and splicing within the stalk, CD44 can range from 80 to 200 kDa (1). Within the N-terminal invariant portion of the ECD (aa 21-222), human CD44 shares 76%, 76%, 86%, 83% and 79% identity with corresponding mouse, rat, equine, canine and bovine CD44, respectively. The many reported functions of CD44 fall within three categories (1). First, CD44 binds hyaluronan and other ligands within the extracellular matrix and can function as a "platform" for growth factors and metalloproteinases. Second, CD44 can function as a co-receptor that modifies activity of receptors including MET and the ERBB family of tyrosine kinases. Third, the CD44 intracellular domain links the plasma membrane to the actin cytoskeleton via the ERM proteins, ezrin, radixin and moesin. CD44 can be synthesized in a soluble form (8) or may be cleaved at multiple sites by either membrane-type matrix metalloproteinases, or ADAM proteases to produce soluble ectodomains (9-10). The cellular portion may then undergo gamma secretase-dependent intramembrane cleavage to form an A beta-like transmembrane portion and a cytoplasmic signaling portion that affects gene expression (11‑12). These cleavage events are thought to promote metastasis by enhancing tumor cell motility and growth (1, 8). CD44v6 plays an important role in colorectal cancer progression involving in cell colonization, invasion, and metastasis and is considered a functional cancer biomarker (7). Our Avi-tag Biotinylated human CD44v6 Fc Chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

Item	Value
References	Ponta, H. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:33. Screaton, G.R. et al. (1992) Proc. Natl. Acad. Sci. USA 89:12160. Screaton, G.R. et al. (1993) J. Biol. Chem. 268:12235. Lynch, K.W. (2004) Nat. Rev. Immunol. 4:931. Todaro, M. et al. (2014) Cell stem cell 14:342. Vizoso, F.J. et al. (2004) J. Cancer Res. Clin. Oncol. 130:679. Ma, L. et al. (2019) Cell Death Dis. 10:30. Yu, Q. and B.P. Toole (1996) J. Biol. Chem. 271:20603. Nagano, O. and H. Saya (2004) Cancer Sci. 95:930. Nakamura, H. et al. (2004) Cancer Res. 64:876. Murakami, D. et al. (2003) Oncogene 22:1511. Lammich, S. et al. (2002) J. Biol. Chem. 277:44754.
Entrez Gene IDs	960 (Human); 12505 (Mouse); 25406 (Rat); 100126860 (Porcine)
Alternate Names	CD44, CD44 antigen, CD44 molecule (Indian blood group), CD44R, CDw44, CSPG8, ECMR-III, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HCAM, HCELL, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, IN, LHR, MC56, MDU2, MDU2CD44 antigen (homing function and Indian blood group system), MDU3, MDU3CDW44, MIC4, MIC4MGC10468, MUTCH-I, PGP-1, PGP-I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I, cell surface glycoprotein CD44, chondroitin sulfate proteoglycan 8, epican, hematopoietic cell E- and L-selectin ligand, homing function and Indian blood group system

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Recombinant Human CD44v6 Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI11175

Key Product Details

Product Specifications for Recombinant Human CD44v6 Fc Chimera Avi-tag Protein, CF

Scientific Data Examples for Recombinant Human CD44v6 Fc Chimera Avi-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: CD44

Customer Reviews for Recombinant Human CD44v6 Fc Chimera Avi-tag Protein, CF

Product Documents for Recombinant Human CD44v6 Fc Chimera Avi-tag Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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