Recombinant Human CD8 alpha/beta Heterodimer Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9358-CD

Analyzed by SEC-MALS.
R&D Systems, part of Bio-Techne
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Key Product Details

Source

HEK293

Accession #

P01732 (CD8 alpha ) & P10966 (CD8 beta)

Structure / Form

Disulfide-linked heterodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all CD8 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CD8 protein
Human CD8 alpha
(Ser22-Asp182)
Accession # P01732		 Acidic Tail HHHHHH
Human CD8 beta
(Asn19-Pro170)
Accession # P10966		 Basic Tail FLAG
(DYKDDDDK)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser22 (CD8 alpha ) & Asn19 (CD8 beta )

Predicted Molecular Mass

26 kDa (CD8 alpha ) & 26 kDa (CD8 beta )

SDS-PAGE

32-43 kDa, reducing conditions

Activity

Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. Bechard, D. et al. (2001) J. Immunol. 167:3099.
The ED50 for this effect is 0.8-4 μg/mL.

Scientific Data Images for Recombinant Human CD8 alpha/beta Heterodimer Protein, CF

Recombinant Human CD8 alpha/beta Heterodimer Protein SEC-MALS.

Recombinant Human CD8 alpha/beta (Catalog # 9358-CD) has a molecular weight (MW) of 60.3 kDa as analyzed by SEC-MALS, suggesting that this protein is a heterodimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Recombinant Human CD8 alpha/beta Heterodimer Protein Binding Activity

Recombinant Human CD8 alpha/beta Heterodimer Protein Binding Activity

Immobilized Recombinant Human CD8 alpha/beta Heterodimer (Catalog # 9358-CD) supports the adhesion of Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.8-4 μg/mL.

Formulation, Preparation and Storage

9358-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD8

CD8, also known as Ly2 or Leu2, is a heterodimeric glycoprotein (alpha and beta subunits) that functions in conjunction with the T cell receptor in the recognition of MHC class I/peptide complexes (1, 2). CD8 alpha is expressed on double positive (CD4+ CD8+) thymocytes and mature CD8+ cytolytic T cells (CTL) (3-5), intraepithelial lymphocytes (IEL) (6), some gamma delta T cells (7), subsets of thymic and splenic dendritic cells (DC) (8), and peritoneal mast cells (9). It can form disulfide linked homodimers or heterodimers with CD8 beta (10). Thymic CD8+ DC express primarily alpha beta heterodimers, while splenic CD8+ DC primarily express alpha alpha homodimers (8). CD8 alpha+ DC can present viral antigenic peptides in complex with MHC I and prime CTL responses (11). The approximately 35 kDa mature mouse CD8 alpha consists of a 169 amino acid (aa) extracellular domain (ECD) with one Iglike domain, a 21 aa transmembrane segment, and a 30 aa cytoplasmic domain (12). Within the ECD, mouse CD8 alpha shares 49% and 64% aa sequence identity with human and rat CD8 alpha, respectively.

References

  1. Laugel, B. et al. (2011) J. Leukoc. Biol. 90:1089.
  2. Cole, D.K. et al. (2012) Immunology 137:139.
  3. Germain, R.N. (2002) Nat. Rev. Immunol. 2:309.
  4. Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
  5. Nakayama, K. et al. (1994) Science 263:1131.
  6. Wang, J. and J.R. Klein (1994) Science 265:1860.
  7. MacDonald, H.R. et al. (1990) Eur. J. Immunol. 20:927.
  8. Vremec, D. et al. (1992) J. Exp. Med. 176:47.
  9. Lin, T.J. et al. (1998) J. Immunol. 161:6265.
  10. Snow, P.M. and C. Terhorst (1983) J. Biol. Chem. 258:14675.
  11. Belz, G.T. et al. (2004) J. Immunol. 172:1996.
  12. Nakauchi, H. et al. (1985) Proc. Natl. Acad. Sci. USA 82:5126.

Alternate Names

CD8, CD8A

Entrez Gene IDs

925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)

Gene Symbol

CD8A

UniProt

P01732 (CD8 alpha ) & P10966 (CD8 beta)

Additional CD8 Products

Product Documents for Recombinant Human CD8 alpha/beta Heterodimer Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human CD8 alpha/beta Heterodimer Protein, CF

For research use only

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