Recombinant Human CD8 alpha Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI10927

Biotinylated
R&D Systems, part of Bio-Techne
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

P01732.1

Structure / Form

Biotinylated via Avi-tag.

Conjugate

Biotin

Applications

Bioactivity

View all CD8 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CD8 protein
Human CD8 alpha
(Ser22-Asp182)
Accession # P01732.1		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser22

Predicted Molecular Mass

46 kDa

SDS-PAGE

55-61 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Human CD8 alpha Antibody (Catalog # MAB3803) is immobilized at 2 μg/mL, 100 μg/mL, Biotinylated Recombinant Human CD8 alpha Fc Chimera Avi-tag binds with an ED50 of 2.00-12.0 ng/mL.

Scientific Data Images for Recombinant Human CD8 alpha Fc Chimera Avi-tag Protein, CF

When Human CD8 alpha Antibody (Catalog # MAB3803) is immobilized at 2 μg/mL, 100 μg/mL, Biotinylated Recombinant Human CD8 alpha Fc Chimera Avi-tag (AVI10927) binds with an ED50 of 2.00-12.0 ng/mL.

Biotinylated Recombinant Human CD8 alpha Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human CD8 alpha Fc Chimera Avi-tag Protein (Catalog # AVI10927) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-61 kDa and 110-120 kDa, respectively.

Formulation, Preparation and Storage

AVI10927
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD8

CD8, also known as Ly2 or Leu2, is a heterodimeric glycoprotein (alpha and beta subunits) that functions in conjunction with the T cell receptor in the recognition of MHC class I/peptide complexes (1, 2). CD8 alpha is expressed on double positive (CD4+ CD8+) thymocytes and mature CD8+ cytolytic T cells (CTL) (3-5), intraepithelial lymphocytes (IEL) (6), some gamma delta T cells (7), subsets of thymic and splenic dendritic cells (DC) (8), and peritoneal mast cells (9). It can form disulfide linked homodimers or heterodimers with CD8 beta (10). Thymic CD8+ DC express primarily alpha beta heterodimers, while splenic CD8+ DC primarily express alpha alpha homodimers (8). CD8 alpha+ DC can present viral antigenic peptides in complex with MHC I and prime CTL responses (11). The approximately 35 kDa mature mouse CD8 alpha consists of a 169 amino acid (aa) extracellular domain (ECD) with one Iglike domain, a 21 aa transmembrane segment, and a 30 aa cytoplasmic domain (12). Within the ECD, mouse CD8 alpha shares 49% and 64% aa sequence identity with human and rat CD8 alpha, respectively. Our Avi-tag Biotinylated human CD8 alpha features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of bionylation and the rest of the protein is unchanged so there is no interference in the protein bioactivity.

References

  1. Laugel, B. et al. (2011) J. Leukoc. Biol. 90:1089.
  2. Cole, D.K. et al. (2012) Immunology 137:139.
  3. Germain, R.N. (2002) Nat. Rev. Immunol. 2:309.
  4. Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
  5. Nakayama, K. et al. (1994) Science 263:1131.
  6. Wang, J. and J.R. Klein (1994) Science 265:1860.
  7. MacDonald, H.R. et al. (1990) Eur. J. Immunol. 20:927.
  8. Vremec, D. et al. (1992) J. Exp. Med. 176:47.
  9. Lin, T.J. et al. (1998) J. Immunol. 161:6265.
  10. Snow, P.M. and C. Terhorst (1983) J. Biol. Chem. 258:14675.
  11. Belz, G.T. et al. (2004) J. Immunol. 172:1996.
  12. Nakauchi, H. et al. (1985) Proc. Natl. Acad. Sci. USA 82:5126.

Alternate Names

CD8, CD8A

Entrez Gene IDs

925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)

Gene Symbol

CD8A

UniProt

P01732.1

Additional CD8 Products

Product Documents for Recombinant Human CD8 alpha Fc Chimera Avi-tag Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human CD8 alpha Fc Chimera Avi-tag Protein, CF

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