Recombinant Human CD82 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 4616-CD

R&D Systems, part of Bio-Techne
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Key Product Details

Source

HEK293

Accession #

P27701

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all CD82/Kai-1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CD82/Kai-1 protein
MD
 Human IgG1
(Pro100-Lys330)		 IEGR Human CD82
(Gly103-Gln225)
Accession # P27701


N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

40.7 kDa

SDS-PAGE

53-65 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant CD82/Kai‑1 Fc Chimera is used at 5 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human TIMP-1 that produces 50% optimal binding response is 2.5-12.5 μg/mL. 

Scientific Data Images for Recombinant Human CD82 Fc Chimera Protein, CF

Recombinant Human CD82 Fc Chimera Protein SDS-PAGE

Recombinant Human CD82 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human CD82/Kai-1 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 53-65 kDa and 105-130 kDa, respectively.

Formulation, Preparation and Storage

4616-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: CD82/Kai-1

CD82 antigen, also known as Kai-1, C33 and TSPAN27, is a widely expressed palmitoylated molecule of the tetraspanin superfamily (1, 2). The general architecture of tetraspanins contain four transmembrane domains, two extracellular loops, a small inner cytoplasmic loop, and intra-cellular N and C termini (1). One of the extracellular loops is termed the large extracellular loop (LEL) and it is responsible for interactions with binding partners such as integrins. Within the LEL, human CD82/Kai-1 shares 65% and 63%amino acid (aa) sequence identity with mouse and rat CD82/Kai-1, respectively. CD82/Kai-1 is a component of the promiscuous TIMP-1 interacting protein complex on the cell surface of human adenocarcinoma cells and gives insight into tumorigenic metastatic potential (3). CD82/Kai-1 suppresses EMT in prostate cancer cells adhered to fibronectin leading to reduced cell migration and invasiveness (4). CD82/Kai-1 function is important for muscle stem cell function in muscular disorders (5). Overexpression of CD82/Kai-1 suppresses growth, migration and invasion of oral cancer cells and may be considered as a potential therapeutic target in oral cancer (6).

References

  1. Mazurov, D. et al. (2006) J. Biol. Chem. 282:3896.
  2. Berditchevski, F. et al. 2001, J Cell Sci. 114:4143.
  3. Zang, J. et al. (2017) Oncotarget. 8:6496.
  4. Lee, J. et al. (2017) Oncotarget.  8:1641.
  5. Alexander, MS. et al. (2016) Cell Stem Cell. 19:800.
  6. Chai, J. et al. (2017) Mol Med. Rep. April; 15:1527.

Alternate Names

CD82, IA4, KAI1, SAR2, ST6, TSPAN27

Entrez Gene IDs

3732 (Human); 12521 (Mouse); 83628 (Rat)

Gene Symbol

CD82

UniProt

P27701

Additional CD82/Kai-1 Products

Product Documents for Recombinant Human CD82 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human CD82 Fc Chimera Protein, CF

For research use only

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