Recombinant Human CEACAM-3/CD66d Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9985-CM

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
9985-CM-050
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

CHO

Accession #

P40198

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all CEACAM-3/CD66d Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CEACAM-3/CD66d protein
Human CEACAM-3
(Lys35-Gly155)
Accession # P40198		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys35

Predicted Molecular Mass

39.7 kDa

SDS-PAGE

48-54 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CEACAM‑3/CD66d Fc Chimera is immobilized at 1 µg/mL (100 µL/well), Recombinant Human CEACAM-7 (Catalog # 9010-CM) binds with an ED50 of 0.05-0.4 μg/mL.

Scientific Data Images for Recombinant Human CEACAM-3/CD66d Fc Chimera Protein, CF

Recombinant Human CEACAM-3/CD66d Fc Chimera Protein Binding Activity

Recombinant Human CEACAM-3/CD66d Fc Chimera Protein Binding Activity

When Recombinant Human CEACAM-3/CD66d Fc Chimera (Catalog # 9985-CM) is immobilized at 1 µg/mL, Recombinant Human CEACAM-7 (Catalog # 9010-CM) binds with an ED50 of 0.05-0.4 µg/mL.
Recombinant Human CEACAM-3/CD66d Fc Chimera Protein SDS-PAGE

Recombinant Human CEACAM-3/CD66d Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human CEACAM‑3 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 48-54 kDa and 96-110 kDa, respectively.

Formulation, Preparation and Storage

9985-CM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: CEACAM-3/CD66d

Carcinoembryonic Antigen-related Cell Adhesion Molecule 3 (CEACAM-3), or CD66d, is part of the CEA protein family consisting of CEACAMs and the pregnancy-specific glycoproteins (PSGs). Both CEACAM and PSG molecules have been identified in humans and belong to the much larger glycosylphosphatidylinositol (GPI)-linked immunoglobulin (Ig) superfamily (1, 2). Human CEACAM-3 is ~35 kDa, consisting of an extracellular domain (ECD) containing one IgV-like domain, a single transmembrane domain and a cytoplasmic tail. The cytoplasmic tail of CEACAM-3 contains an immunoreceptor tyrosine-based activation motif (ITAM), which recruits kinases to propagate pro-inflammatory signaling cascades (3). Interestingly, CEACAM-3 appears to be primate specific, with on non-primate orthologs currently identified (4). Originally discovered as a biomarker for colorectal cancer (5), CEACAMs have now been associated with numerous intracellular signaling processes including cell adhesion, cell growth, recognition and differentiation, angiogenesis, and apoptosis (6-8). Unlike other CEA family members, CEACAM-3 has not been shown to form cell–cell adhesion interactions with other CEACAM family members (9). CEACAM-3 has been found to be specifically expressed on human neutrophils and other granulocytes and appears to be a specific adaptation of the innate immune system to cope with a small set of host-specific pathogen (9). CEACAM-3 was identified as critical for opsonin-independent phagocytosis and bacterial clearance (10). CEACAM-3 binds to the colony opacity-associated (Opa) outer membrane proteins of bacteria, such as Neisseria gonorrhoeae, and triggers uptake of the pathogen and subsequent elimination (9, 10).

References

  1. Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
  2. Zebhauser R, et al. (2005) Genomics 86:566.
  3. Schmitter, T. et al. (2004) J. Exp. Med. 199:35.
  4. Pavlopoulou A. and Scorilas A. (2014) Genome Biol Evol. 6:1314.
  5. Gold P and Freedman, S.O. (1965) J Exp Med. 122:467.
  6. Obrink, B. (1997) Curr Opin Cell Biol. 9:616.
  7. Horst, AK. and Wagener, C. (2004) Handb Exp Pharmacol. 165:283.
  8. Kuespert K et al. (2006) Curr Opin Cell Biol. 18:565.
  9. Pils S. et al. (2008) Int J Med Microbiol. 298:553.
  10. Schmitter, T. et al. (2004) J. Exp. Med. 199:35.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 3

Alternate Names

CD66d, CGM1

Entrez Gene IDs

1084 (Human); 384557 (Mouse); 24926 (Rat)

Gene Symbol

CEACAM3

UniProt

P40198

Additional CEACAM-3/CD66d Products

Product Documents for Recombinant Human CEACAM-3/CD66d Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human CEACAM-3/CD66d Fc Chimera Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.