Recombinant Human CEACAM-7 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9010-CM

R&D Systems, part of Bio-Techne
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9010-CM-050
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Key Product Details

Source

CHO

Accession #

Q14002

Conjugate

Unconjugated

Applications

Bioactivity

View all CEACAM-7 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CEACAM-7 protein
Thr36-Asn233, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr36

Predicted Molecular Mass

23 kDa

SDS-PAGE

42-52 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CEACAM-7 is immobilized at 0.1 µg/mL (100 µL/well), the concentration of Recombinant Human
CEACAM-1/CD66a (Catalog # 2244-CM) that produces 50% of the optimal binding response is approximately 1.6-8 ng/mL.

Formulation, Preparation and Storage

9010-CM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage

Background: CEACAM-7

Carcinoembryonic antigen-related cell adhesion molecule 7 (CEACAM-7), also known as CGM2, is an approximately 40 kDa GPI-anchored glycoprotein in the CEACAM family of adhesion molecules (1). Mature human CEACAM-7 consists of two Ig-like domains followed by the GPI anchor (2). Alternative splicing generates a short isoform that lacks the second Ig-like domain. CEACAM-7 is preferentially expressed on the luminal surface of epithelial cells near the mouth of colonic crypts and on pancreatic ductal epithelial cells (3, 4). It is down-regulated during colorectal adenoma progression (2-6) but can be up-regulated during the development of gastric carcinoma (7). R&D Systems in-house testing indicates that CEACAM-7 binds to CEACAM-1, consistent with the heterophilic interaction of CEACAM-1 with other CEACAM family members (1, 8, 9).

References

  1. Tchoupa, A.K. et al. (2014) Cell Commun. Signal. 12:27.
  2. Thompson, J. et al. (1994) J. Biol. Chem. 269:32924.
  3. Thompson, J. et al. (1997) Cancer Res. 57:1776.
  4. Scholzel, S. et al. (2000) Am. J. Pathol. 156:595.
  5. Nollau, P. et al. (1997) Cancer Res. 57:2354.
  6. Nollau, P. et al. (1997) Am. J. Pathol. 151:521.
  7. Zhou, J. et al. (2011) World J. Surgical Oncol. 9:172.
  8. Markel, G. et al. (2004) J. Immunol. 173:3732.
  9. Oikawa, S. et al. (1992) Biochem. Biophys. Res. Commun. 186:881.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 7

Alternate Names

CEACAM7, CGM2

Entrez Gene IDs

1087 (Human)

Gene Symbol

CEACAM7

UniProt

Q14002 (Human)

Additional CEACAM-7 Products

Product Documents for Recombinant Human CEACAM-7 Protein, CF

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Certificate of Analysis

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Product Specific Notices for Recombinant Human CEACAM-7 Protein, CF

For research use only

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