Recombinant Human CEACAM-3/CD66d His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9868-CM

R&D Systems, part of Bio-Techne
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9868-CM-050
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Key Product Details

Source

NS0

Accession #

P40198-1

Conjugate

Unconjugated

Applications

Bioactivity

View all CEACAM-3/CD66d Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CEACAM-3/CD66d protein
Lys35-Gly155, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys35

Predicted Molecular Mass

14 kDa

SDS-PAGE

19-25 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CEACAM‑3/CD66d is immobilized at 2 µg/mL (100 µL/well), the concentration of Recombinant Human CEACAM-7 (Catalog # 9010-CM) that produces 50% of the optimal binding response is 0.6-3.6 μg/mL.

Scientific Data Images for Recombinant Human CEACAM-3/CD66d His-tag Protein, CF

Recombinant Human CEACAM-3/CD66d His-tag Protein Binding Activity

Recombinant Human CEACAM-3/CD66d His-tag Protein Binding Activity

When Recombinant Human CEACAM-3/CD66d (Catalog # 9868-CM) is coated at 2 µg/mL, Recombinant Human CEACAM-7 (Catalog # 9010-CM) binds with an ED50 of 0.3-3.6 µg/mL.
Recombinant Human CEACAM-3/CD66d His-tag Protein SDS-PAGE

Recombinant Human CEACAM-3/CD66d His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human CEACAM‑3 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 19-25 kDa.

Formulation, Preparation and Storage

9868-CM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: CEACAM-3/CD66d

Carcinoembryonic Antigen-related Cell Adhesion Molecule 3 (CEACAM-3), or CD66d, is part of the CEA protein family consisting of CEACAMs and the pregnancy-specific glycoproteins (PSGs). Both CEACAM and PSG molecules have been identified in humans and belong to the much larger glycosylphosphatidylinositol (GPI)‑linked immunoglobulin (Ig) superfamily (1, 2). Mature human CEACAM-3 is approximately 35 kDa, consisting of an extracellular domain (ECD) containing one IgV-like domain, a single transmembrane domain and a cytoplasmic tail. The cytoplasmic tail of CEACAM-3 contains an immunoreceptor tyrosine-based activation motif (ITAM), which recruits kinases to propagate pro-inflammatory signaling cascades (3). CEACAM-3 appears to be primate specific, with no non-primate orthologs currently identified (4). Originally discovered as a biomarker for colorectal cancer (5), CEACAMs have now been associated with numerous intracellular signaling processes including cell adhesion, cell growth, recognition and differentiation, angiogenesis, and apoptosis (6-8). Unlike other CEA family members, CEACAM-3 has not been shown to form cell–cell adhesion interactions with other CEACAM family members (9). CEACAM-3 has been found to be specifically expressed on human neutrophils and other granulocytes and appears to be ann adaptation of the innate immune system to handle specific host pathogens (9). The granulocyte-specific CEACAM epitope is present on at least four CEA family members, CEACAM-1 -3, -6 and -8, which upon engagement on the neutrophil surface triggers a transient activation signal that requires extracellular calcium and regulates the adhesive activity of the beta2 integrin, CD11/CD18 (10, 11). CEACAM-3 is critical for opsonin‑independent phagocytosis and bacterial clearance (3). CEACAM-3 binds to the colony opacity-associated (Opa) outer membrane proteins of bacteria, such as Neisseria gonorrhoeae, and triggers uptake of the pathogen and subsequent elimination (3, 9).

References

  1. Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
  2. Zebhauser, R. et al. (2005) Genomics 86:566.
  3. Schmitter, T. et al. (2004) J. Exp. Med. 199:35.
  4. Pavlopoulou A. and Scorilas A. 2014 Genome Biol Evol. 6(6):1314.
  5. Gold P and Freedman SO, (1965) J Exp Med 122:467.
  6. Obrink, B. (1997) Curr Opin Cell Biol 9:616.
  7. Horst, AK. and Wagener, C. (2004) Handb Exp Pharmacol 283.
  8. Kuespert K et al. (2006) Curr Opin Cell Biol. 18(5):565.
  9. Pils S. et al. (2008) Int. J. of Med. Micro. 298, 7–8:553.
  10. Nagel G. et al. (1993) Eur J Biochem 214:27.
  11. Skubitz KM et al. (1996) J Leukoc Biol 60:106.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 3

Alternate Names

CD66d, CGM1

Entrez Gene IDs

1084 (Human); 384557 (Mouse); 24926 (Rat)

Gene Symbol

CEACAM3

UniProt

P40198-1

Additional CEACAM-3/CD66d Products

Product Documents for Recombinant Human CEACAM-3/CD66d His-tag Protein, CF

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Product Specific Notices for Recombinant Human CEACAM-3/CD66d His-tag Protein, CF

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