Recombinant Human CHST10 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6140-ST

R&D Systems, part of Bio-Techne
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6140-ST-020
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Key Product Details

Source

CHO

Accession #

O43529

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Carbohydrate Sulfotransferase 10/CHST10 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Carbohydrate Sulfotransferase 10/CHST10 protein
Pro32-Asn356, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Pro32

Predicted Molecular Mass

39 kDa

SDS-PAGE

55-75 kDa, reducing conditions

Activity

Measured by its ability to transfer sulfate from PAPS to phenolpthalein glucuronic acid.
The specific activity is >350 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

6140-ST
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Carbohydrate Sulfotransferase 10/CHST10

CHST10 is the only known sulfotransferase that catalyzes the transfer of sulfate to position 3 of terminal glucuronic acid of both protein- and lipid-linked oligosaccharides (1, 2). CHST10 is a key enzymes for the synthesis of HNK-1 carbohydrate structure, a sulfated glucuronyl-lactosaminyl residue carried by many neural recognition molecules (3). HNK-1 is involved in cell interactions during ontogenetic development and in synaptic plasticity in the adult. In adults, CHST10 is highly expressed in brain, testis and ovary. Recently, it is reported that CHST10 functions as a suppressor of invasiveness of human melanoma cells (4). Like many other carbohydrate specific sulfotransferases, CHST10 is a single-pass type II membrane protein and is localized in Golgi apparatus membrane. The recombinant human CHST10 only contains the luminal enzymatic domain and the enzymatic activity is measured using a phosphatase-coupled assay (5).

References

  1. Ong, E. et al. (1999) J. Biol. Chem. 274:25608.
  2. Ong, E. et al. (1998) J. Biol. Chem. 273:5190.
  3. Kizuka, Y. et al. (2006) J. Biol. Chem. 281:13644.
  4. Zhao, X. et al. (2009) Cancer Res. 69:5218.
  5. Prather, B. et al. (2012) Anal. Biochem. 423:86.

Alternate Names

CHST10, HNK1ST

Entrez Gene IDs

9486 (Human)

Gene Symbol

CHST10

UniProt

O43529

Additional Carbohydrate Sulfotransferase 10/CHST10 Products

Product Documents for Recombinant Human CHST10 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human CHST10 Protein, CF

For research use only

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