Recombinant Human cIAP-1 (HIAP-2) Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 818-IA
Key Product Details
Product Specifications
Source
E. coli-derived human cIAP-1/HIAP-2 protein
| ATVID | 10-His tag | SIEGRA | Human cIAP-1 (His2-Ser618) Accession # Q13490 |
| N-terminus | C-terminus | ||
Purity
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
N-terminal Sequence Analysis
Ala
Predicted Molecular Mass
72 kDa
SDS-PAGE
68 kDa, reducing conditions
Activity
Measured by its ability to inhibit DEVD-AFC cleavage activity in cell extracts activated by addition of cytochrome c and dATP.
The IC50 for this effect is 25-1000 nM.
Optimal dilutions should be determined by each laboratory for each application.
The IC50 for this effect is 25-1000 nM.
Optimal dilutions should be determined by each laboratory for each application.
Formulation, Preparation and Storage
818-IA
| Formulation | Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: cIAP-1/HIAP-2
cIAP-1 (also known as MIHB and HIAP-2) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. cIAP-1 has 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). cIAP-1 inhibits caspases by interaction of the BIR domain with the active caspase. Caspase activity may be restored through interactions with the Reaper like motif on mitochondrial proteins such as SMAC/Diablo or HTRA-2/Omi. cIAP-1 is reported to be cleaved by caspases in fetal rat hepatocytes treated with TGF-beta.
References
- Roy, N. et al. (1997) EMBO J. 23:6914.
- Deveraux, Q. et al. (1997) Nature 388:300.
- Deveraux, Q. and J. Reed (1999) Genes & Develop. 13:239.
- Herrera, B. et al. (2002) FEBS Letters 520:93.
Long Name
Cellular Inhibitor of Apoptosis Protein 1
Alternate Names
BIRC2, cIAP1, HIAP-2, MIHB
Gene Symbol
BIRC2
UniProt
Additional cIAP-1/HIAP-2 Products
Product Documents for Recombinant Human cIAP-1 (HIAP-2) Protein, CF
Product Specific Notices for Recombinant Human cIAP-1 (HIAP-2) Protein, CF
For research use only
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