Recombinant Human CLEC9a Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6049-CL

R&D Systems, part of Bio-Techne
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6049-CL-050
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Key Product Details

Source

NS0

Accession #

Q6UXN8

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all CLEC9a Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CLEC9a protein
Met-Asp
 Human IgG1
(Pro100-Lys330)
 IEGR Human CLEC9a
(Lys57-Val241)
Accession # Q6UXN8
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

47.8 kDa (monomer)

SDS-PAGE

55-65 kDa, reducing conditions

Activity

Measured by flow cytometry for its ability to bind a ligand expressed in FasL-treated mouse B cells. Sancho, D. et al. (2009) Nature 458:899.
At 100 ng/mL, >50% of necrotic cells bind to Recombinant Human CLEC9a.

Formulation, Preparation and Storage

6049-CL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC9a

CLEC9A (C-type lectin domain family 9 member A), also known as DNGR-1, is a type II transmembrane glycoprotein member of the C-type lectin superfamily (1). Mature mouse CLEC9A consists of a 35 amino acid (aa) cytoplasmic domain with an ITAM-like motif, a 21 aa transmembrane segment, and a 185 extracellular domain (ECD) that contains a stalk region and one C-type lectin domain (CTLD) (2-4). Within the ECD, human CLEC9A shares 57% aa sequence identity with mouse and rat CLEC9A. Although the CTLD of CLEC9A structurally resembles that of other C-type lectins, it lacks the conserved residues that typically mediate calcium and carbohydrate binding. CLEC9A is expressed as a disulfide-linked homodimer of approximately 50 kDa N-glycosylated subunits (2, 4). Human CLEC9A expression is restricted to a subpopulation of BDCA-3+ conventional dendritic cells (cDC) and CD16- monocytes (2-5). BDCA-3+ cDC are analagous to mouse CD8+ cDC which are specialized in antigenic cross-presentation in complex with MHC class I molecules (6). In mouse, CLEC9A is additionally expressed on plasmacytoid dendritic cells (3, 4). CLEC9A ligation enhances antigen uptake and processing, leading to presentation on MHC class I and cytotoxic T cell (CTL) priming (2-5, 7). Its recognition of filamentous actin in dead cells is important for triggering an immune response to necrotic cell debris (8-10). CLEC9A is also required for the presentation of viral proteins and the subsequent CTL-mediated killing of virus-infected cells (11, 12). CLEC9A signaling triggers activation of the tyrosine kinase Syk (2, 8).

References

  1. Kerrigan, A.M. and G.D. Brown (2010) Immunol. Rev. 234:335.
  2. Huysamen, C. et al. (2008) J. Biol. Chem. 283:16693.
  3. Caminschi, I. et al. (2008) Blood 112:3264.
  4. Sancho, D. et al. (2008) J. Clin. Invest. 118:2098.
  5. Schreibelt, G. et al. (2012) Blood 119:2284.
  6. Dudziak, D. et al. (2007) Science 315:107.
  7. Poulin, L.F. et al. (2010) J. Exp. Med. 207:1261.
  8. Sancho, D. et al. (2009) Nature 458:899.
  9. Ahrens, S. et al. (2012) Immunity 36:635.
  10. Zhang, J.-G. et al. (2012) Immunity 36:646.
  11. Iborra, S. et al. (2012) J. Clin. Invest. 122:1628.
  12. Zelenay, S. et al. (2012) J. Clin. Invest. 122:1615.

    Long Name

    C-type Lectin-like Receptor 9

    Alternate Names

    CD370, DNGR1

    Entrez Gene IDs

    283420 (Human); 232414 (Mouse)

    Gene Symbol

    CLEC9A

    UniProt

    Q6UXN8

    Additional CLEC9a Products

    Product Documents for Recombinant Human CLEC9a Fc Chimera Protein, CF

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    Product Specific Notices for Recombinant Human CLEC9a Fc Chimera Protein, CF

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