Recombinant Human Collagen XV alpha 1/COL15A1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9854-CL

R&D Systems, part of Bio-Techne
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Key Product Details

Source

HEK293

Accession #

P39059

Structure / Form

Noncovalently-linked homotrimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Collagen XV alpha 1/COL15A1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Collagen XV alpha 1/COL15A1 protein
Hemagglutinin Tag
(YPYDVPDYA)
 Human COL15A1
(Asn1133-Ser1381)
Accession # P39059


Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr

Predicted Molecular Mass

29 kDa

SDS-PAGE

26-36 kDa, reducing conditions

Activity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Human Collagen XV  alpha1/COL15A1, immobilized at 0.5 μg/mL, is able to significantly enhance neurite outgrowth.

Formulation, Preparation and Storage

9854-CL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Collagen XV alpha 1/COL15A1

Recombinant human Collagen XV alpha1/COL15A1 is the C-terminal endostatin-like domain (amino acids 1133-1381) of human Collagen XV. Collagen XV is one of the two members of a vertebrate collagen family termed multiplexins. The other member of this family is Collagen XVIII. This family is characterized by a collagen triple helix region that is interrupted by several non-collagenous stretches, as well as a C-terminal non-triple helical NC1 domain (1, 2). The NC1 domain is composed of three functionally distinct regions: an association region in its most N-terminal part that trimerizes NC1 monomers, a protease-sensitive hinge region, and the C-terminal globular endostatin (ES) domain, or in the case of Collagen XV, an ES-like domain (2, 3). Both Collagen XV and Collagen XVIII are located in the basement membrane or the basement zone. They show anti-angiogenic and anti-tumoral properties (4), and play an important role in tissue homeostasis in liver, kidney, eye and the central nervous system (5). The Drosophila multiplexin (Dmp) is shown to modulate axon pathfinding accuracy in motor pathways (1). Within amino acids 1133-1381, the human protein shares 74% sequence homology with its mouse counterpart, and 73% sequence homology with that of the rat. This ES-like domain in Collagen XV show approximately 60% sequence identity with the endostatin domain of type XVIII Collagen. Studies have shown that endostatins derived from Collagens XV and XVIII differ in structural and binding properties, tissue distribution and anti-angiogenic activity (6).

References

  1. Meyer, F. and Moussian B. (2009) Develop. Growth Differ. 51:483.
  2. Marneros, A. G. and Olsen B. R. (2001) Matrix Biol. 20:337.
  3. Wirz, J. et al. (2011) Matrix Biol. 30:9.
  4. Ricard-Blum, S. and Ballut L. (2011) Front Biosci 16:674.
  5. Heljasvaara, R. et al. (2016) Matrix Biol. 57-58:55.
  6. Sasaki, T. et al. (2000) J. Mol. Biol. 301:1179.

Long Name

Collagen Type XV Alpha 1 Chain

Alternate Names

COL15A1, Collagen 15 alpha 1, Collagen Type XV Proteoglycan, Collagen XV, Alpha-1 Polypeptide, Endostatin-XV, Restin

Entrez Gene IDs

1306 (Human); 12819 (Mouse); 298069 (Rat)

Gene Symbol

COL15A1

UniProt

P39059

Additional Collagen XV alpha 1/COL15A1 Products

Product Documents for Recombinant Human Collagen XV alpha 1/COL15A1 Protein, CF

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Product Specific Notices for Recombinant Human Collagen XV alpha 1/COL15A1 Protein, CF

For research use only

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