Recombinant Human Collectrin Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7580-CL

R&D Systems, part of Bio-Techne
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7580-CL-050
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Key Product Details

Source

CHO

Accession #

Q9HBJ8

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Collectrin Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Collectrin protein
Human Collectrin
(Glu15-Pro141)
Accession # Q9HBJ8		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu15

Predicted Molecular Mass

41 kDa (monomer)

SDS-PAGE

54-64 kDa, reducing conditions

Activity

Measured by its ability to inhibit proliferation of mIMCD-3 mouse epithelial cells.
The ED50 for this effect is 1.0-4.0 μg/mL.

Formulation, Preparation and Storage

7580-CL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Collectrin

Collectrin, also known as TMEM27 and NX‑17, is a 35‑45 kDa transmembrane protein that shows structural similarity to the C‑terminal region of Angiotensin Converting Enzyme 2 (ACE‑2) but lacks peptidase activity (1, 2). Mature human Collectrin consists of a 127 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 60 aa cytoplasmic domain (3). Within the ECD, human Collectrin shares 87% and 89% aa sequence identity with mouse and rat Collectrin, respectively. Collectrin is expressed as a disulfide‑linked homodimer of variably glycosylated subunits (4‑6). It is highly expressed in the proximal convoluted tubules of renal collecting ducts and inhibits the proliferation of tubule epithelial cells (3, 6‑8). It associates with several amino acid transporters on the luminal surface of these cells, where it is required for the reabsorption of multiple amino acids (7‑9). Collectrin is also expressed in pancreatic islet beta cells and enhances glucose‑stimulated insulin secretion as well as insulin resistance (4, 5, 9, 10). In both the kidney and pancreas, cytoplasmic Collectrin interacts with multiple components of the SNARE complex and other vesicle trafficking proteins (5, 6). A 25 kDa glycosylated fragment of the ECD can be shed from both renal tubule cells and pancreatic beta cells (4, 10). In beta cells, shedding is mediated by BACE2, and the portion left in the membrane can be subsequently cleaved by gamma‑Secretase, releasing the intracellular domain into the cytosol (11). Inhibition of Collectrin shedding from beta cells results in increased beta cell mass and insulin secretion (11).

References

  1. Lambert, D.W. et al. (2010) Cell. Mol. Life Sci. 67:89.
  2. Zhang, Y. and J. Wada (2007) Biochem. Biophys. Res. Commun. 363:1.
  3. Zhang, H. et al. (2001) J. Biol. Chem. 276:17132.
  4. Akpinar, P. et al. (2005) Cell Metab. 2:385.
  5. Fukui, K. et al. (2005) Cell Metab. 2:373.
  6. Zhang, Y. et al. (2007) PLoS ONE 5:e414.
  7. Danilczyk, U. et al. (2006) Nature 444:1088.
  8. Malakauskas, S.M. et al. (2007) Am. J. Physiol. Renal Physiol. 292:F533.
  9. Malakauskas, S.M. et al. (2009) Mol. Endocrinol. 23:881.
  10. Altirriba, J. et al. (2010) Diabetologia 53:1406.
  11. Esterhazy, D. et al. (2011) Cell Metab. 14:365.

Alternate Names

NX17, TMEM27

Entrez Gene IDs

57393 (Human); 57394 (Mouse); 57395 (Rat)

Gene Symbol

CLTRN

UniProt

Q9HBJ8

Additional Collectrin Products

Product Documents for Recombinant Human Collectrin Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human Collectrin Fc Chimera Protein, CF

For research use only

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