Recombinant Human COMP His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 3134-CPB

R&D Systems, part of Bio-Techne
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3134-CPB-050
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Key Product Details

Source

NS0

Accession #

P49747

Structure / Form

Disulfide linked homopentamer

Conjugate

Unconjugated

Applications

Bioactivity

View all COMP/Thrombospondin-5 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human COMP/Thrombospondin-5 protein
Gln21-Ala757 (Ala256Arg), with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln21

Predicted Molecular Mass

81.8 kDa

SDS-PAGE

106-118 kDa, reducing conditions

Activity

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.
Recombinant Human COMP/Thrombospondin‑5 immobilized at 10 µg/mL (100 µL/well) will induce more than 40% of ATDC-5 cell adhesion.

Scientific Data Images for Recombinant Human COMP His-tag Protein, CF

Recombinant Human COMP His-tag Protein Bioactivity

Recombinant Human COMP His-tag Protein Bioactivity

Immobilized Recombinant Human COMP/Thrombospondin‑5 (Catalog # 3134-CPB) at 10 µg/mL (100 µL/well) induces more than 40% cell adhesion.
Recombinant Human COMP His-tag Protein SDS-PAGE

Recombinant Human COMP His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human COMP/Thrombospondin‑5 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 106-118 kDa and 500 - 550 kDa, respectively.

Formulation, Preparation and Storage

3134-CPB
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: COMP/Thrombospondin-5

Cartilage Oligomeric Matrix Protein (COMP), also known as Thrombospondin-5, is a 110 kDa multidomain calcium binding protein that associates with other extracellular matrix molecules. Thrombospondin-1 and -2 constitute subgroup A and form homotrimers, whereas Thrombospondin-3, -4, and COMP constitute subgroup B and form homopentamers (1-4). The human COMP cDNA encodes a 757 amino acid (aa) precursor that includes a 20 aa signal sequence followed by a non-collagenous coiled-coil domain, four EGF-like repeats, seven TSP type-3 repeats, and a globular TSP C-terminal domain (5). Human COMP shares 86-93% aa sequence identity with rat, mouse, equine, bovine, and canine COMP. Within the TSP type-3 repeats and TSP C-terminal domain, human COMP shares 60%, 61%, 74%, and 80% aa sequence identity with human Thrombospondin-1, -2, -3, and -4, respectively. The coiled coil domain mediates the association of COMP into disulfide-linked homopentamers with a central hub and peripheral globular domains connected by flexible strands (6, 7). An axial pore is formed by the coiled coil assembly and binds vitamin D3 which is involved in bone and cartilage metabolism (8). An RGD sequence in the third TSP type-3 repeat mediates chondrocyte attachment via Integrin  alpha5 beta1, although when reduced and in the absence of calcium, attachment is mediated via Integrin alphaV beta3 (9). COMP is upregulated in rheumatoid arthritis and osteoarthritis, hepatocellular carcinomas, chronic pancreatitis, and pancreatic carcinomas (10-12). Elevated circulating COMP levels are used as a biomarker for early onset of some skeletal disorders (10). Several mutations are associated with skeletal dysplasias, and the most common, a point mutation in the third TSP type-3 repeat, results in diminished calcium binding ability (13, 14).

References

  1. Adams, J.C. and J. Lawler (2004) Int J. Biochem. Cell Biol. 36:961.
  2. Posey, K.L. et al. (2004) Int. J. Biochem. Cell Biol. 36:1005.
  3. Adams, J.C. (2004) Int. J. Biochem. Cell Biol. 36:1102.
  4. Mann, H.H. et al. (2004) J. Biol. Chem. 279:25294.
  5. Newton, G. et al. (1994) Genomics, 24:435.
  6. DiCesare, P. et al. (1995) J. Orthopaedic Res. 13:422.
  7. Efimov, V.P. et al. (1994) FEBS Lett. 341:54.
  8. Ozbek, S. et al. (2002) EMBO J. 21:5960.
  9. Chen, F.H. et al. (2005) J. Biol. Chem. 280:32655.
  10. Wislowska, M. and B. Jablonska (2005) Clin. Rheumatol. 24:278.
  11. Xiao, Y. et al. (2004) J. Gastroenterol. Hepatol. 19:296.
  12. Liao, Q. et al. (2003) Scand. J. Gastroenterol. 38:207.
  13. Kennedy, J. et al. (2005) Eur. J. Hum. Genet. 13:547.
  14. Hou, J. et al. (2000) Cell Calcium 27:309.

Long Name

Cartilage Oligomeric Matrix Protein

Alternate Names

EDM1, EPD1, MED, PSACH, Thrombospondin-5, Thrombospondin5

Entrez Gene IDs

1311 (Human); 12845 (Mouse)

Gene Symbol

COMP

UniProt

P49747

Additional COMP/Thrombospondin-5 Products

Product Documents for Recombinant Human COMP His-tag Protein, CF

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Product Specific Notices for Recombinant Human COMP His-tag Protein, CF

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