Recombinant Human Complement Component C1s Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2060-SE
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked heterodimer
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Complement Component C1s protein
Met1-Asp688, with a C-terminal 6-His tag
Met1-Asp688, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Glu16 (A chain) & Ile438 (B chain)
Predicted Molecular Mass
47 kDa (A chain) & 28 kDa (B chain)
SDS-PAGE
62 kDa and 32 kDa, reducing conditions
Activity
Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.
The specific activity is >20,000 pmol/min/µg, as measured under the described conditions.
The specific activity is >20,000 pmol/min/µg, as measured under the described conditions.
Reviewed Applications
Read 1 review rated 5 using 2060-SE in the following applications:
Formulation, Preparation and Storage
2060-SE
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl, CaCl2 and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Complement Component C1s
The classical complement pathway plays a major role in innate immunity against infection. This pathway is triggered by C1, a multimolecular complex composed of the recognition protein C1q and two serine proteases, C1r and C1s. Following the C1q recognition, C1r is autoactivated, and in turn activates C1s, which cleaves C4 and C2, the C1 substrates (1). Both C1r and C1s activation involve cleavage of a specific Arg-Ile bond, converting single-chain proenzymes into active proteases of disulfide bond-linked chains (A and B) (2). The A chains contain multiple domains in the order of CUB1-EGF-CUB2-CCP1-CCP2-Activation Peptide. The B chains contain the serine protease catalytic domain. The full-length (amino acid residues 1-688) of human C1s was expressed (3-5). The purified protein corresponded to the processed active form, with A and B chains starting at residue Glu16 and Ile438, respectively.
References
- Arlaud, G.J. et al. (2002) Biochem. Soc. Trans. 30:1001.
- Lacroix, M. et al. (2001) J. Biol. Chem. 276:36233.
- Tosi, M. et al. (1987) Biochemistry 26:8516.
- Mackinnon, C.M. et al. (1987) Eur. J. Biochem. 169:547.
- Kusumoto, H. et al. (1988) Proc. Natl. Acad. Sci. USA 85:7307.
Alternate Names
C1S
Entrez Gene IDs
716 (Human)
Gene Symbol
C1S
UniProt
Additional Complement Component C1s Products
Product Documents for Recombinant Human Complement Component C1s Protein, CF
Product Specific Notices for Recombinant Human Complement Component C1s Protein, CF
For research use only
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