Recombinant Human Complement Factor H-related 4/CFHR4, CF

R&D Systems, part of Bio-Techne | Catalog # 11300-CH

HEK293, His-tag
R&D Systems, part of Bio-Techne
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11300-CH-050
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Key Product Details

Source

HEK293

Accession #

Q92496.3

Conjugate

Unconjugated

Applications

Bioactivity

View all Complement Factor H-related 4/CFHR4 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Complement Factor H-related 4/CFHR4 protein
Glu20-Glu578, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu20

Predicted Molecular Mass

64 kDa

SDS-PAGE

92-103 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Complement Component C3d His-tag (Catalog # 2655-C3) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human Complement Factor H-related 4/CFHR4 His-tag (Catalog # 11300-CH) binds with an ED50 of 1.50-15.0 ng/mL.

Scientific Data Images for Recombinant Human Complement Factor H-related 4/CFHR4, CF

Recombinant Human Complement Factor H‑related 4/CFHR4 His-tag Protein Binding Activity.

When Recombinant Mouse Complement Component C3d His-tag (2655-C3) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human Complement Factor H‑related 4/CFHR4 His-tag Protein (Catalog # 11300-CH) binds with an ED50 of 1.50-15.0 ng/mL.

Recombinant Human Complement Factor H-related 4/CFHR4 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human Complement Factor H-related 4/CFHR4 His-tag Protein (Catalog # 11300-CH) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 92-103 kDa.

Formulation, Preparation and Storage

11300-CH
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Factor H-related 4/CFHR4

Complement factor H-related protein 4 (CFHR4) is a secreted glycoprotein member of the factor H family of glycoproteins (1). The human complement factor H protein family consists of the complement and immune regulators factor H, the factor H-like protein 1 (FHL-1) and five factor H-related proteins (FHR-1 to -5) (1). The genes of this family are located on human chromosome 1q32, which is known as the regulator of complement activation (RCA) gene clusters (2). CFHRs are exclusively composed of individually folded protein domains, termed short consensus repeats (SCRs) or complement control modules. All CFHRs, including CFHR4, are capable of binding complement factor C3b, discriminate between self and non-self cell surfaces, and have been proposed to deregulate complement activation by inhibiting interaction of CFH with C3b and C3d (1, 3). CFHR4 activates complement through its interaction with C3b (4). CFHR4 can also bind to native (pentameric) C-reactive protein (pCRP) through its first SCR domain and is suggested to promote pCRP binding to necrotic cells surfaces, thus promoting cell clearance (5).  CFHR4 is synthesized as 2 isoforms by hepatocytes and circulates in plasma. The shorter isoform, FHR4B, contains 5 of 9 SCRs. The CFHR4 product includes a duplication insertion of 247 aa and represents the longer alternative splice form termed FHR4A (6). 

References

  1. Skerka, C. et al. (2013) Mol. Immunol. 56:170.
  2. Diaz-Guillen, M.A. et al. (1999) Immunogenetics 49:549.
  3. Hellwage, J. et al. (1999) FEBS Lett. 462:345.
  4. Hebecker, M. and J. Jozsi (2011) J. Biol. Chem. 287:19528.
  5. Mihlan, M. et al. (2009) Mol. Immunol. 46:335.
  6. Jozsi, M. et al. (2005) Eur. J. Hum. Genet. 13:321.

Alternate Names

CFHL4, CFHR4, Complement Factor Hrelated 4, FHR4

Entrez Gene IDs

10877 (Human)

Gene Symbol

CFHR4

UniProt

Q92496.3

Additional Complement Factor H-related 4/CFHR4 Products

Product Documents for Recombinant Human Complement Factor H-related 4/CFHR4, CF

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Product Specific Notices for Recombinant Human Complement Factor H-related 4/CFHR4, CF

For research use only

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