Recombinant Human Complement Factor H-related 4/CFHR4, CF

R&D Systems, part of Bio-Techne | Catalog # 5980-CH

Sf21, His-tag
R&D Systems, part of Bio-Techne
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Key Product Details

Source

Sf 21 (baculovirus)

Accession #

CAA66980

Conjugate

Unconjugated

Applications

Bioactivity

View all Complement Factor H-related 4/CFHR4 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Complement Factor H-related 4/CFHR4 protein
Gln19-Glu331, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln19 inferred from enzymatic pyroglutamate treatment revealing Glu20.

Predicted Molecular Mass

37 kDa

SDS-PAGE

39-45 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Complement Component C3d (Catalog # 2655-C3) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human Complement Factor H‑related 4/CFHR4 binds with an ED50 of 1-6 ng/mL.

Scientific Data Images for Recombinant Human Complement Factor H-related 4/CFHR4, CF

Recombinant Human Complement Factor H-related 4/CFHR4 Binding Activity

Recombinant Human Complement Factor H-related 4/CFHR4 Binding Activity

When Recombinant Mouse Complement Component C3d (Catalog # 2655-C3) is immobilized at 2 µg/mL, 100 µL/well, Recombinant Human Complement Factor H-related 4/CFHR4 (Catalog # 5980-CH) binds with an ED50 of 1-6 ng/mL.
Recombinant Human Complement Factor H-related 4/CFHR4 SDS-PAGE

Recombinant Human Complement Factor H-related 4/CFHR4 SDS-PAGE

2 μg/lane of Recombinant Human Complement Factor H‑related 4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at ~42 kDa and ~40 kDa, respectively.

Formulation, Preparation and Storage

5980-CH
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: Complement Factor H-related 4/CFHR4

Complement factor H-related protein 4 (CFHR4) is a secreted glycoprotein member of the factor H family of glycoproteins (1). The human complement factor H protein family consists of the complement and immune regulators factor H, the factor H-like protein 1 (FHL-1) and five factor H-related proteins (FHR-1 to -5) (1). The genes of this family are located on human chromosome 1q32, which is known as the regulator of complement activation (RCA) gene clusters (2). CFHRs are exclusively composed of individually folded protein domains, termed short consensus repeats (SCRs) or complement control modules. All CFHRs, including CFHR4, are capable of binding complement factor C3b, discriminate between self and non-self cell surfaces, and have been proposed to deregulate complement activation by inhibiting interaction of CFH with C3b and C3d (1, 3). CFHR4 activates complement through its interaction with C3b (4). CFHR4 can also bind to native (pentameric) C-reactive protein (pCRP) through its first SCR domain and is suggested to promote pCRP binding to necrotic cells surfaces, thus promoting cell clearance (5). CFHR4 is synthesized as 2 isoforms by hepatocytes and circulates in plasma. The longer 86 kDa alternative splice form termed FHR4A includes a duplication insertion of 247 aa. The CFHR4 product represents the shorter isoform, FHR4B, containing 5 of 9 SCRs (6).

References

  1. Skerka, C. et al. (2013) Mol. Immunol. 56:170.
  2. Diaz-Guillen, M.A. et al. (1999) Immunogenetics 49:549.
  3. Hellwage, J. et al. (1999) FEBS Lett. 462:345.
  4. Hebecker, M. and J. Jozsi (2011) J. Biol. Chem. 287:19528.
  5. Mihlan, M. et al. (2009) Mol. Immunol. 46:335.
  6. Jozsi, M. et al. (2005) Eur. J. Hum. Genet. 13:321.

Alternate Names

CFHL4, CFHR4, Complement Factor Hrelated 4, FHR4

Entrez Gene IDs

10877 (Human)

Gene Symbol

CFHR4

UniProt

CAA66980

Additional Complement Factor H-related 4/CFHR4 Products

Product Documents for Recombinant Human Complement Factor H-related 4/CFHR4, CF

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Product Specific Notices for Recombinant Human Complement Factor H-related 4/CFHR4, CF

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