Recombinant Human Complement Factor H-related 5/CFHR5, CF
R&D Systems, part of Bio-Techne | Catalog # 3845-F5
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Complement Factor H-related 5/CFHR5 protein
Glu19-Glu569, with a C-terminal 10-His tag
Glu19-Glu569, with a C-terminal 10-His tag
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Glu19
Predicted Molecular Mass
64 kDa
SDS-PAGE
73 kDa, reducing conditions
Activity
Measured by its ability to inhibit the binding of biotinylated Recombinant Human Complement Factor H-related 5/CFHR5 to immobilized Recombinant Human C-Reactive Protein/CRP (Catalog # 1707-CR). McRae, J.L. et al. (2005) J. Immunol. 174:6250.
Formulation, Preparation and Storage
3845-F5
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris and NaCl. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Complement Factor H-related 5/CFHR5
The human complement factor H protein family consists of the complement and immune regulators factor H, the factor H-like protein 1 (FHL-1) and five factor H-related proteins (FHR-1 to -5) (2). Members of this family are exclusively composed of individually folded protein domains, termed short consensus repeats (SCRs) or complement control modules. The genes of this family have been located in human chromosome 1q32, which is known as the regulator of complement activation (RCA) gene clusters (3). FHR-5 has been identified initially as a universal component of complement deposits (1), and detected in glomerular immune deposits (4). The pattern of deposits is similar to other complement components, suggesting that FHR-5 may play a role in complement activation and regulation. It is synthesized in the liver and consists of 9 SCRs. Its biological function is not understood fully. FHR-5 exhibits similar characteristics as those of factor H in heparin binding, CRP binding, and lipoprotein association (5). Weak factor I-dependent cofactor activity for C3b cleavage has also been observed (5).
References
- McRae, J. L. et al. (2001) J. Biol. Chem. 276:6747.
- Zipfel, P. F. et al. (2002) Biochem. Soc. Trans. 30:971.
- McRae, J. L. et al. (2002) Genetica. 114:157.
- Murphy, B. et al. (2002) Am. J. Kidney. Dis. 39:24.
- McRae, J. L. et al. (2005) J. Immunol. 174:6250.
Alternate Names
CFHL5, CFHR5, Complement Factor Hrelated 5, FHR5
Entrez Gene IDs
81494 (Human)
Gene Symbol
CFHR5
UniProt
Additional Complement Factor H-related 5/CFHR5 Products
Product Documents for Recombinant Human Complement Factor H-related 5/CFHR5, CF
Product Specific Notices for Recombinant Human Complement Factor H-related 5/CFHR5, CF
For research use only
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