Recombinant Human Contactin-6 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7394-CN

R&D Systems, part of Bio-Techne
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7394-CN-050
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Key Product Details

Source

CHO

Accession #

Q9UQ52

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Contactin-6 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Contactin-6 protein
Human Contactin-6
(Asp20-Ser891)
Accession # Q9UQ52		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp20

Predicted Molecular Mass

122.9 kDa

SDS-PAGE

130-170 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of Neuro-2A mouse neuroblastoma cells.
The ED50 for this efffect is 1.0-4.0 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

7394-CN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Contactin-6

Contactin-6, also known as NB-3, is a 130 kDa member of the TAG/F3 subgroup of Contactin GPI‑linked neuronal adhesion proteins (1, 2). Mature human Contactin-6 consists of six immunoglobulin‑like domains (aa 26‑587), a flexible linker region, four fibronectin type III domains (aa 597‑992), and a GPI anchor (3). Within aa 20‑891, human Contactin-6 shares 89% aa sequence identity with mouse and rat Contactin-6. Contactin-6 expression is upregulated postnatally in the cerebellum, brainstem, and hippocampus, and it shows differential expression within various structures of the brain (3‑7). In the cerebellum, it is presynaptically localized at synapses between excitatory glutamatergic parallel fibers and Purkinje cells (7). Contactin-6 associates in cis with CHL-1, PTPR alpha, and PTPR gamma as well as in trans with Notch-1 on oligodendrocytes (6, 8‑10). Its binding to Notch-1 triggers the release and translocation of the Notch intracellular domain to the nucleus and promotes oligodendrogenesis (6). Contactin-6 function is important in the postnatal development and function of the cerebellum, as shown in knockout mice which exhibit poor motor coordination (5, 7).

References

  1. Shimoda, Y. and K. Watanabe (2009) Cell Adhesion Migration 3:64.
  2. Stoeckli, E.T. (2010) Cell Adh. Migr. 4:523.
  3. Kamei, Y. et al. (1998) J. Neurosci. Res. 51:275.
  4. Lee, S. et al. (2000) Gene 245:253.
  5. Takeda, Y. et al. (2003) J. Neurobiol. 56:252.
  6. Cui, X.-Y. et al. (2004) J. Biol. Chem. 279:25858.
  7. Sakurai, K. et al. (2009) Dev. Neurobiol. 69:811.
  8. Ye, H. et al. (2008) EMBO J. 27:188.
  9. Bouyain, S. and D.J. Watkins (2010) Proc. Natl. Acad. Sci. 107:2443.
  10. Ye, H. et al. (2011) J. Biol. Chem. 286:26071.

Alternate Names

CNTN6, Contactin6, NB3

Entrez Gene IDs

27255 (Human); 53870 (Mouse); 27256 (Rat)

Gene Symbol

CNTN6

UniProt

Q9UQ52 (Human)

Additional Contactin-6 Products

Product Documents for Recombinant Human Contactin-6 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Contactin-6 Fc Chimera Protein, CF

For research use only

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