Recombinant Human CRTAC1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5234-CR

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
5234-CR-050
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

NS0

Accession #

Q9NQ79

Structure / Form

Monomer

Conjugate

Unconjugated

Applications

Bioactivity

View all CRTAC1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CRTAC1 protein
Ser28-Cys661, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser28

Predicted Molecular Mass

69 kDa

SDS-PAGE

83-90 kDa, reducing conditions

Activity

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.
The ED50 for this effect is 0.25-1.5 μg/mL.

Reviewed Applications

Read 1 review rated 4 using 5234-CR in the following applications:

Formulation, Preparation and Storage

5234-CR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRTAC1

Cartilage acidic protein 1 (CRTAC1), also known as CEP‑68, is a 95 kDa glycosylated protein that contains four FG‑GAP domains and one EGF‑like domain. An integrin‑binding RGD motif is present in the human protein but absent in the mouse and rat proteins. Mature human CRTAC1 shares 89% amino acid sequence identity with mouse and rat CRTAC. It is secreted by chondrocytes into the extracellular matrix of cartilage (1, 2). It is also up‑regulated in type II alveolar epithelial cells upon dexamethasone exposure (3). CRTAC1B, otherwise known as LOTUS, is an 85 kDa alternatively spliced isoform that has a substituted C‑terminal region with a potential transmembrane segment (2, 4). CRTAC1B is preferentially expressed in select regions of the brain on neuronal growth cones and neurite shafts (2, 4). It associates with the Nogo Receptor (NgR) and prevents Nogo‑induced growth cone collapse (4, 5). In mouse, CRTAC1B plays an important role in axonal bundling and formation of the lateral olfactory tract (4).

References

  1. Steck, E. et al. (2001) Biochem. J. 353:169.
  2. Steck, E. et al. (2007) Matrix Biol. 26:30.
  3. Ballard, P.L. et al. (2010) Am. J. Physiol. Lung Cell. Mol. Physiol. 299:L36.
  4. Sato, Y. et al. (2011) Science 333:769.
  5. Kurihara, Y. et al. (2012) Biochem. Biophys. Res. Commun. 418:390.

Long Name

Cartilage Acidic Protein 1

Alternate Names

ASPIC1, CEP68

Entrez Gene IDs

55118 (Human); 72832 (Mouse); 171438 (Rat)

Gene Symbol

CRTAC1

UniProt

Q9NQ79

Additional CRTAC1 Products

Product Documents for Recombinant Human CRTAC1 Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human CRTAC1 Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.