Recombinant Human CUL1/RBX1 Neddylated Complex Protein, CF

R&D Systems, part of Bio-Techne | Catalog # E3-411

R&D Systems, part of Bio-Techne
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E3-411-025
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Key Product Details

Source

Sf 21 (baculovirus)

Accession #

Q13616.2 (CUL1), P62877.1 (RBX1)

Conjugate

Unconjugated

Applications

Bioactivity

View all CUL1/RBX1 Complex Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CUL1/RBX1 Complex protein
Met1 - Ala776 with a N-terminal 10-His Sumo tag (CUL1), Met1 - His108 (RBX1)

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

104 kDa (neddylated CUL1), 12 kDa (RBX1)

Activity

Typical enzyme concentration to support in vitro conjugation will depend on experimental conditions.

Formulation, Preparation and Storage

E3-411
Formulation Supplied as a solution in HEPES, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: CUL1/RBX1 Complex

Cullin-1 (CUL1) is a core component of multiple SCF (SKP1-CUL1-F-box) E3 Ubiquitin ligase complexes that mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription.  In the SCF complex, CUL1 serves as a scaffold that organizes the SKP1-F-box recognition subunits with the RBX1 subunit and contributes to catalysis through positioning of the substrate and an E2 ubiquitin-conjugating enzyme.  In vivo, the E3 ubiquitin ligase activity of the SCF complex is dependent on neddylation of the cullin subunit, though neddylation may be dispensable for some in vitro reactions.  

References

  1. Baek K., et al. (2020) Nature 578: 461.
  2. Duda D.M., et al.  (2008) Cell 134: 995.
  3. Duda D.M., et al.  (2012) Mol. Cell 47: 371.
  4. Goldenberg S.J., et al.  (2004) Cell  119: 517.
  5. Zheng N., et al.  (2002) Nature 416: 703.

Long Name

Cullin-1/RING Box Protein 1 Complex

Entrez Gene IDs

8454 (Human)

Gene Symbol

CUL1

UniProt

Q13616.2 (CUL1), P62877.1 (RBX1)

Additional CUL1/RBX1 Complex Products

Product Documents for Recombinant Human CUL1/RBX1 Neddylated Complex Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human CUL1/RBX1 Neddylated Complex Protein, CF

For research use only

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