Recombinant Human CUL2/RBX1 Neddylated Complex Protein, CF

R&D Systems, part of Bio-Techne | Catalog # E3-421

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R&D Systems, part of Bio-Techne
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E3-421-025
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Key Product Details

Source

Sf 21 (baculovirus)

Accession #

Q13617.2 (CUL2), P62877.1 (RBX1)

Conjugate

Unconjugated

Applications

Bioactivity

View all CUL2/RBX1 Complex Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CUL2/RBX1 Complex protein
Met1 - Ala745 with a N-terminal 10-His Sumo tag (CUL2), Met1 - His108 (RBX1)

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

102 kDa (neddylated CUL2), 12 kDa (RBX1)

Activity

Typical enzyme concentration to support in vitro conjugation will depend on experimental conditions.

Formulation, Preparation and Storage

E3-421
Formulation Supplied as a solution in HEPES, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: CUL2/RBX1 Complex

Cullin-2 is a core component of multiple cullin-RING based "ECS" (Elongin B/C-CUL2-SOCS-box protein) E3 Ubiquitin ligase complexes that mediate the ubiquitination of proteins involved in a variety of cellular processes.  In the ECS complex, Cullin-2 serves as a scaffold that organizes the ELOB/C-associated recognition subunits with the RBX1 subunit and contributes to the catalysis through positioning of the substrate and an E2 ubiquitin-conjugating enzyme.  In vivo, the E3 ubiquitin ligase activity of the ECS complex is dependent on neddylation of the cullin subunit, though neddylation may be dispensable for some in vitro reactions. 

References

  1. Baek K., et al. (2020) Nature 578: 461.
  2. Duda D.M., et al. (2008) Cell  134: 995.
  3. Duda D.M., et al. (2012) Mol. Cell  47: 371.
  4. Goldenberg S.J., et al. (2004) Cell  119: 517.
  5. Zheng N., et al. (2002) Nature  416: 703.

Long Name

Cullin-2/RING Box Protein 1 Complex

Entrez Gene IDs

8453 (Human)

Gene Symbol

CUL2

UniProt

Q13617.2 (CUL2), P62877.1 (RBX1)

Additional CUL2/RBX1 Complex Products

Product Documents for Recombinant Human CUL2/RBX1 Neddylated Complex Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human CUL2/RBX1 Neddylated Complex Protein, CF

For research use only

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