Recombinant Human Cyclophilin A Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 3589-CAB
Key Product Details
Product Specifications
Source
E. coli-derived human Cyclophilin A protein
Met1-Glu165
Met1-Glu165
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met1
Predicted Molecular Mass
18 kDa
SDS-PAGE
17 kDa, reducing conditions
Activity
Measured by its ability to inhibit calcineurin phosphatase activity in the presence of Cyclosporin A.
The IC50 for inhibition of calcineurin activity is <700 nM, as measured under the described conditions.
The IC50 for inhibition of calcineurin activity is <700 nM, as measured under the described conditions.
Formulation, Preparation and Storage
3589-CAB
| Formulation | Supplied as a 0.2 μm filtered solution in MES and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Cyclophilin A
Cyclophilin A, also called Peptidyl-prolyl Isomerase A, PPIA, CYPA, and CYPH, was originally characterized for its ability to catalyze the transition between cis- and trans- proline residues critical for proper folding of proteins (1). Cyclophilin is also incorporated into many viruses, including HIV-1, where it has been speculated to be involved in functions such as viral assembly and infectivity (2). The immunosuppressive activity of cyclosporins has been correlated with their ability to form complexes with cyclophilins that inhibit calcineurin phosphatase activity (3) and prevent incorporation of cyclophilin into viral particles (4). The cyclosporin/cyclophilin complex selectively binds and inactivates calcineurin (3, 5), making it a useful inhibitor for studying calcineurin activity.
References
- Hamilton, G.S. and J.P. Steiner (1998) J. Med. Chem. 41:5119.
- Cantin, R. et al. (2005) J. Virology 79:6577.
- Liu, J. et al. (1992) Biochemistry 31:3896.
- Wiegers K. and H.G. Krausslich (2002) Virology 294:289.
- Liu, J. et al. (1991) Cell 66:807.
Alternate Names
CYPA, CYPH, PPIA, Rotamase
Gene Symbol
PPIA
UniProt
Additional Cyclophilin A Products
Product Documents for Recombinant Human Cyclophilin A Protein, CF
Product Specific Notices for Recombinant Human Cyclophilin A Protein, CF
For research use only
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