Recombinant Human Cystatin F Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1889-PI
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Inhibition Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Cystatin F protein
Gly20-His145, with a C-terminal 6-His tag
Gly20-His145, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Gly20
Predicted Molecular Mass
15 kDa
SDS-PAGE
16 kDa, 21 kDa and 26 kDa, reducing conditions
Activity
Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC (Catalog # ES008).
The IC50 value is <6.0 nM, as measured under the described conditions.
The IC50 value is <6.0 nM, as measured under the described conditions.
Formulation, Preparation and Storage
1889-PI
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris and NaCl. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 25 mM Tris and 150 mM NaCl, pH 7.5. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Cystatin F
Cystatin F, also known as leukocystatin and CMAP (Cystatin‑like Metastasis‑Associated Protein), is a member of the cystatin superfamily (1‑3). Cystatin F is selectively expressed by hematopoietic cells and may be a biomarker for both liver metastasis and inflammatory lung disorders (3, 4). As a cysteine protease inhibitor, it shows selectivity towards cathepsin L and legumain (1, 2, 5). Compared to other secreted cystatins including C, D, E/M, S, SA and SN, which contain two intra disulfide bonds, cystatin F has two extra Cys residues that may be involved in inter disulfide bonds. Indeed, rhCystatin F showed disulfide bond‑linked dimer formation, which was also the case for an E. coli expressed fusion protein containing mature human cystatin F and glutathione S‑transferase (2).
References
- Ni, J. et al. (1998) J. Biol. Chem. 273:24797.
- Halfon, S. et al. (1998) J. Biol. Chem. 273:16400.
- Utsunomiya, T. et al. (2002) Clin Cancer 8:2591
- Werle, B. et al. (2003) Biol. Chem. 384:281.
- Gruninger-Leitch, F. et al. (2000) Nat. Biotechnol. 18:66.
Alternate Names
CMAP, CST7, Cystatin 7, Leukocystatin
Entrez Gene IDs
8530 (Human)
Gene Symbol
CST7
UniProt
Additional Cystatin F Products
Product Documents for Recombinant Human Cystatin F Protein, CF
Product Specific Notices for Recombinant Human Cystatin F Protein, CF
For research use only
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