Recombinant Human Cystatin SA Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1201-PI
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Cystatin SA protein
Trp21-Ala141, with a C-terminal 10-His tag
Trp21-Ala141, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Trp21
Predicted Molecular Mass
16 kDa
SDS-PAGE
16 kDa, reducing conditions
Activity
Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC (Catalog # ES008).
The IC50 value is<8.0 nM, as measured under the described conditions.
The IC50 value is<8.0 nM, as measured under the described conditions.
Formulation, Preparation and Storage
1201-PI
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris and NaCl. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 25 mM Tris and 150 mM NaCl, pH 8.0. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Cystatin SA
Cystatin SA is a member of family 2 of the cystatin superfamily (1). Together with cystatins S and SN, it is produced by the salivary gland and secreted largely in the submandibular/sublingual saliva (2). Cystatin SA inhibits cathepsin L, but may not inhibit cathepsins B and H (3). The functions of Cystatin SA are largely unknown. However, it has been suggested to play a role in the control of periodontal tissue destruction (3).
References
- Abrahamson, M. (1994) Methods Enzymol. 244:685.
- Baron, A.C. et al. (1999) Exp. Oral. Biol. 5:344.
- Baron, A. et al. (1999) Oral Dis. 5:234.
Alternate Names
CST2, CSTSA
Entrez Gene IDs
1470 (Human)
Gene Symbol
CST2
UniProt
Additional Cystatin SA Products
Product Documents for Recombinant Human Cystatin SA Protein, CF
Product Specific Notices for Recombinant Human Cystatin SA Protein, CF
For research use only
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