Recombinant Human DC-SIGN/CD209 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11265-DC

CHO-Expressed
R&D Systems, part of Bio-Techne
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11265-DC-050
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Key Product Details

Source

CHO

Accession #

Q9NNX6.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all DC-SIGN/CD209 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human DC-SIGN/CD209 protein
MD Human IgG1
(Pro100-Lys330)		 IEGR Human DC-SIGN/CD209
(Lys62-Ala404)
Accession # Q9NNX6.1
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

65.7 kDa

SDS-PAGE

62-74 kDa, under reducing conditions.

Activity

Measured by the ability of the immobilized protein to support the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells.
The ED50 for this effect is 0.700-7.00 µg/mL.

Scientific Data Images for Recombinant Human DC-SIGN/CD209 Fc Chimera Protein, CF

Recombinant Human DC-SIGN/CD209 Fc Chimera Protein Bioactivity.

Recombinant Human DC‑SIGN/CD209 Fc Chimera (Catalog # 11265-DC) supports the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells. The ED50 for this effect is 0.700-7.00 µg/mL.

Recombinant Human DC‑SIGN/CD209 Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human DC‑SIGN/CD209 Fc Chimera Protein (Catalog # 11265-DC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 62-74 kDa and 120-150 kDa, respectively.

Formulation, Preparation and Storage

11265-DC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DC-SIGN/CD209

Human Dendritic Cell-specific ICAM-3 Grabbing Non-integrin (DC-SIGN)/CD209 is a member of the C-type lectin family (1). The canonical DC-SIGN/CD209 isoform is a 46 kDa, 404 amino acid (aa) type II transmembrane protein (2). The extracellular region contains a Ca2+-dependent carbohydrate-binding lectin domain (2). Multiple human DC-SIGN/CD209 splice forms exist, generating both membrane-bound and soluble forms (3). DC-SIGN/CD209 is not well conserved between mouse and human, with the extracellular domain sharing only 63% aa identity. The DC-SIGN/CD209 lectin domain binds mannose oligosaccharides on pathogens including HIV as well as self glycoproteins including ICAMs (2, 4). DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation (5, 6).

References

  1. Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
  2. Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356.
  3. Mummidi, S. et al. (2001) J. Biol. Chem. 276:33196.
  4. Anthony, R.M. et al. (2008) Proc. Natl. Acad. Sci. USA 105:19571.
  5. Geijtenbeek, T.B. et al. (2000) Cell 100:575.
  6. Garcia-Vallejo, J.J. and Y. van Kooyk (2013) Trends Immunol. 34:482.

Long Name

Dendritic Cell-specific ICAM-3-grabbing Non-integrin 1

Alternate Names

CD209, CLEC4L, DC-SIGN1, DCSIGN

Entrez Gene IDs

30835 (Human); 170786 (Mouse); 102121984 (Cynomolgus Monkey)

Gene Symbol

CD209

UniProt

Q9NNX6.1

Additional DC-SIGN/CD209 Products

Product Documents for Recombinant Human DC-SIGN/CD209 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human DC-SIGN/CD209 Fc Chimera Protein, CF

For research use only

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