Recombinant Human DEP-1/CD148 (aa 997-1337) Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1934-DP
Key Product Details
Product Specifications
Source
E. coli-derived human DEP-1/CD148 protein
Arg997-Ala1337, with an N-terminal Met & 6-His tag
Arg997-Ala1337, with an N-terminal Met & 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
40 kDa
SDS-PAGE
37 kDa, reducing conditions
Activity
Measured by its ability to dephosphorylate a tyrosine residue in a peptide containing the EGFR Y992 phosphorylation site (Catalog # ES006).
The specific activity is >100 µmol/min/mg, as measured under the described conditions.
The specific activity is >100 µmol/min/mg, as measured under the described conditions.
Formulation, Preparation and Storage
1934-DP
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl, Glycerol and Betamercaptoethanol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: DEP-1/CD148
Density Enhanced Protein Tyrosine Phosphatase (DEP-1), also known as CD148, HPTP-eta, and PTP receptor type J (PTPRJ), is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. A large (220 kilodalton) glycoprotein found at the cell surface, DEP-1 levels are increased with high cell density (1). DEP-1 phosphatase activity is enhanced by basement membrane proteins (2), suggesting it is involved in regulating cell adhesion and contact interactions. High levels of expression dampen PDGF (3), VEGF (4), and T-cell receptor (5) responses. DEP-1 is widely expressed in tissues, particularly ones forming epithelioid monolayers (6). In the immune system, DEP-1 is found on all cell lineages and is highest on granulocytes (7). Dep-1 is the mutated gene in the Susceptibility to Colon Cancer locus Scc1, which is altered in many human colorectal adenomas (8). Gene knockout mice lacking DEP-1 die at midgestation due to failures in cardiovascular development (9). DEP-1 dephosphorylates a variety of proteins, including the HGF (10), PDGF (11), and VEGF (4) receptors, and beta‑catenin (12). The recombinant protein is the intracellular region of DEP-1 containing the catalytic domain.
References
- Ostman, A. et al. (1994) Proc. Natl. Acad. Sci. USA 91:9680.
- Sorby, M. et al. (2001) Oncogene 20:5219.
- Jandt, E. et al. (2003) Oncogene 22:4175.
- Lampugnani, M.G. et al. (2003) J. Cell Biol. 161:793.
- Baker, J.E. et al. (2001) Mol. Cell. Biol. 21:2393.
- Borges, L.G. et al. (1996) Circ. Res. 79:570.
- de la Fuente-Garcia, M.A. et al. (1998) Blood 91:2800.
- Ruivenkamp, C.A. et al. (2002) Nat. Genet. 31:295.
- Takahashi, T. et al. (2003) Mol. Cell. Biol. 23:1817.
- Palka, H.L. et al. (2003) J. Biol. Chem. 278:5728.
- Kovalenko, M. et al. (2000) J. Biol. Chem. 275:16219.
- Holsinger, L.J. et al. (2002) Oncogene 21:7067.
Long Name
Density Enhanced Receptor Protein Tyrosine Phosphatase
Alternate Names
CD148, DEP1, HPTP-eta, PTPRJ
Gene Symbol
PTPRJ
UniProt
Additional DEP-1/CD148 Products
Product Documents for Recombinant Human DEP-1/CD148 (aa 997-1337) Protein, CF
Product Specific Notices for Recombinant Human DEP-1/CD148 (aa 997-1337) Protein, CF
For research use only
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