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Recombinant Human Desmoglein-3 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11167-DM

R&D Systems, part of Bio-Techne
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11167-DM-050

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Desmoglein-3 protein
Glu24-Lys499, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu24 & Glu50

Predicted Molecular Mass

56 kDa & 53 kDa

SDS-PAGE

57-67 kDa, under reducing conditions.

Activity

Measured by the ability of immobilized Recombinant Human Desmoglein‑3 His-tag (Catalog # 11167-DM)  to support the adhesion of Caki‑2 human clear cell carcinoma epithelial cells. The ED50 for this effect is 0.750-6.00 μg/mL.

Scientific Data Images for Recombinant Human Desmoglein-3 His-tag Protein, CF

Recombinant Human Desmoglein‑3 His-tag Protein Bioactivity.

Measured by the ability of immobilized Recombinant Human Desmoglein‑3 His-tag Protein (Catalog # 11167-DM) to support the adhesion of Caki‑2 human clear cell carcinoma epithelial cells. The ED50 for this effect is 0.750-6.00 μg/mL.

Recombinant Human Desmoglein‑3 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human Desmoglein‑3 His-tag Protein (Catalog # 11167-DM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 57-67 kDa.

Formulation, Preparation and Storage

11167-DM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Desmoglein-3

Desmoglein-3, also known as pemphigus vulgaris antigen (PVA), is a 130 kDa transmembrane glycoprotein that belongs to the cadherin family of calcium dependent adhesion molecules (1 - 3). Human Desmoglein-3 is synthesized with a 26 amino acid (aa) propeptide. The mature protein consists of a 566 aa extracellular domain (ECD) that contains four cadherin-like domains, a 25 aa transmembrane segment, and a 359 aa cytoplasmic domain (4). Within the ECD, human Desmoglein-3 shares 78% aa sequence identity with mouse and rat Desmoglein-3. It shares 44%, 47%, and 56% aa sequence identity with Desmoglein-1, -2, and -4 respectively. Desmoglein-3 is one of the principal components of desmosomes which form adhesive contacts between epithelial cells (1, 2). It is expressed in the basal and suprabasal layers of stratified epithelia in many tissues (4 - 6). During apoptosis, Desmoglein-3 is cleaved by caspases, plus MMP-2, and MMP-9 at sites within the cytoplasmic and extracellular regions, resulting in shortened transmembrane forms and a soluble 75 kDa ECD fragment (7, 8). The downregulation of Desmoglein-3 in oral squamous cell carcinoma correlates with metastatic potential (9). Desmoglein-3 is the target of autoantibodies in pemphigus vulgaris, a blistering skin disorder with compromised inter-keratinocyte adhesion (6, 10). Binding of these antibodies triggers Desmoglein-3 internalization and degradation by keratinocytes (10).

References

  1. Kottke, M.D. et al. (2006) J. Cell Sci. 119:797.
  2. Garrod, D.R. et al. (2002) Mol. Membr. Biol. 19:81.
  3. Leckband, D. and A. Prakasam (2006) Annu. Rev. Biomed. Eng. 8:259.
  4. Amagai, M. et al. (1991) Cell 67:869.
  5. Silos, S.A. et al. (1996) J. Biol. Chem. 271:17504.
  6. Amagai, M. et al (1996) J. Invest. Dermatol. 106:351.
  7. Weiske, J. et al. (2001) J. Biol. Chem. 276:41175.
  8. Cirillo, N. et al. (2007) Oral Dis. 13:341.
  9. Wang, L. et al. (2007) Virchows Arch. Sept. 11 epub.
  10. Calkins, C.C. et al. (2006) J. Biol. Chem. 281:7623.

Alternate Names

CDHF6, Desmoglein3, DSG3

Entrez Gene IDs

1830 (Human); 13512 (Mouse)

Gene Symbol

DSG3

UniProt

Additional Desmoglein-3 Products

Product Documents for Recombinant Human Desmoglein-3 His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Desmoglein-3 His-tag Protein, CF

For research use only

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