Recombinant Human DLL1 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10184-DL

R&D Systems, part of Bio-Techne
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10184-DL-050
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Key Product Details

Source

NS0

Accession #

O00548

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all DLL1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human DLL1 protein
Human DLL1
(Ser22-Glu537)
Accession # O00548		 IEGRMDP Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser22

Predicted Molecular Mass

82 kDa

SDS-PAGE

88-98 kDa, under reducing conditions

Activity

Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842.
The ED50 for this effect is 0.1-0.5 μg/mL.

Scientific Data Images for Recombinant Human DLL1 Fc Chimera Protein, CF

Recombinant Human DLL1 Fc Chimera Protein Bioactivity

Recombinant Human DLL1 Fc Chimera Protein Bioactivity

Recombinant Human DLL1 Fc Chimera (Catalog # 10184-DL) enhances Recombinant Human BMP-2 (Catalog # 355-BM) induced alkaline phosphatase activity in the C3H10T1/2 mouse embryonic fibroblast cell line. The ED50 for this effect is 0.1-0.5 µg/mL.
Recombinant Human DLL1 Fc Chimera Protein SDS-PAGE

Recombinant Human DLL1 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human DLL1 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 88-98 kDa and 170-190 kDa, respectively .

Formulation, Preparation and Storage

10184-DL
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES and EDTA.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DLL1

Delta-like protein 1 (DLL1) is a 90-100 kDa type I transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature human DLL1 consists of a 528 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23 aa transmembrane segment, and a 155 aa cytoplasmic domain (1). Within the ECD, human DLL1 shares 91% aa sequence identity with mouse and rat DLL1. It shares 26%, 37%, and 54% aa sequence identity with DLL2, 3, and 4, respectively. A 60 kDa ECD fragment released by ADAM9, 12, or 17 mediated proteolysis, promotes the proliferation of hematopoietic progenitor cells (2, 3). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent gamma-secretase, enabling the cytoplasmic domain to migrate to the nucleus (4). DLL1 localizes to adherent junctions on neuronal processes through its association with the scaffolding protein MAGI1 (5). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, plus B and T lymphocyte differentiation (6-11). The up-regulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (12). DLL1 is also over-expressed in cervical carcinoma and glioma and contributes to tumor progression (1, 13). Soluble DLL-1 was shown to inhibit differentiation of hematopoietic precursor cells (14).

References

  1. Gray, G.E. et al. (1999) Am. J. Pathol. 154:785.
  2. Dyczynska, E. et al. (2007) J. Biol. Chem. 282:436.
  3. Karanu, F.N. et al. (2001) Blood 97:1960.
  4. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751.
  5. Mizuhara, E. et al. (2005) J. Biol. Chem. 280:26499.
  6. Takahashi, Y. et al. (2003) Development 130:4259.
  7. Teppner, I. et al. (2007) BMC Dev. Biol. 7:68.
  8. Kiernan, A.E. et al. (2005) Development 132:4353.
  9. Schmitt, T.M. and J.C. Zuniga-Pflucker (2002) Immunity 17:749.
  10. Hozumi, K. et al. (2004) Nat. Immunol. 5:638.
  11. Santos, M.A. et al. (2007) Proc. Natl. Acad. Sci. 104:15454.
  12. Limbourg, A. et al. (2007) Circ. Res. 100:363.
  13. Purow, B.W. et al. (2005) Cancer Res. 65:2353.
  14. Han. W. et al. (2000) Blood. 95(5):1616.

Long Name

Delta-like 1

Alternate Names

Delta 1

Entrez Gene IDs

28514 (Human); 13388 (Mouse); 84010 (Rat)

Gene Symbol

DLL1

UniProt

O00548

Additional DLL1 Products

Product Documents for Recombinant Human DLL1 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human DLL1 Fc Chimera Protein, CF

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