Recombinant Human DLL3 His-tag Alexa Fluor® 488 Protein

R&D Systems, part of Bio-Techne | Catalog # AFG9749

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
AFG9749-020
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Learn more about FluorokinesTM Fluorescent-Labeled Proteins

Source

HEK293

Accession #

Q9NYJ7.1

Structure / Form

Labeled with Alexa Fluor® 488 via amines
Excitation Wavelength: 488 nm
Emission Wavelength: 515–545 nm

Conjugate

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Bioactivity

View all DLL3 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human DLL3 protein
Human DLL3
(Ala27-Ala479)
Accession # Q9NYJ7.1		 HP GGGSGGGSGGGS 6-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala27

Predicted Molecular Mass

49 kDa

SDS-PAGE

48-57 kDa, under reducing conditions.

Activity

Measured by flow cytometry for its ability to bind Human DLL3 Antibody conjugated fluorescent beads at 5.00-20.0 µg/mL (100 µL/well).
Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Images for Recombinant Human DLL3 His-tag Alexa Fluor® 488 Protein

Detection of Human DLL3 Antibody with Recombinant Human DLL3 His-tag Alexa Fluor® 488 Protein by Flow Cytometry.

Fluorescent beads conjugated to Human DLL3 Antibody were stained with (A) Recombinant Human DLL3 His-tag Alexa Fluor® 488 (Catalog # AFG9749, filled histogram) or (B) unstained (open histogram).

Recombinant Human DLL3 His-tag Alexa Fluor® 488 Protein SDS-PAGE.

2 μg/lane of Recombinant Human DLL3 His-tag Alexa Fluor® 488 Protein (Catalog # AFG9749) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 48-57 kDa.

Formulation, Preparation and Storage

AFG9749
Formulation Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: DLL3

Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands (1). Mature human DLL3 consists of a 466 amino acid (aa) extracellular domain (ECD) with one DSL domain and six EGF-like repeats, a 21 aa transmembrane segment, and a 105 aa cytoplasmic domain (2). Within the ECD, human DLL3 shares 86% aa sequence identity with mouse and rat DLL3. DLL3 is known as a divergent DSL ligand since it does not function in similar manner as the rest of DSL ligands. It does not activate Notch signaling through trans-activation, but autonomously attenuates signaling induced by other DSL ligands (3). A loss-of-function mutation of DLL3 is linked to axial skeletal defects in the spondylocostal dysplasia, which is linked to abnormal Notch signaling (4). DL-3 promotes proliferation and inhibits apoptosis of cancer cells. The proliferative effect mediated by DLL3 is thought to be due to increased Akt phosphorylation in cancer cells (5).

References

  1. Dunwoodie, S. L. et al. (1997) Development 124:3065.
  2. Bulman, M. P. et al. (2000) Nat. Genet. 24:438.
  3. Ladi, E. et al. (2005) J. Cell Biol. 170:983.
  4. Dunwoodie, S.L. et al. (2002) Development 129:1795.
  5. Deng S.M. et al. (2017) Biochem. Biophys. Res. Commun. 483:488.

Long Name

Delta-like 3

Alternate Names

Pudgy, SCDO1

Entrez Gene IDs

10683 (Human); 13389 (Mouse); 114125 (Rat)

Gene Symbol

DLL3

UniProt

Q9NYJ7.1

Additional DLL3 Products

Product Documents for Recombinant Human DLL3 His-tag Alexa Fluor® 488 Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human DLL3 His-tag Alexa Fluor® 488 Protein


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.