Recombinant Human DNAM-1/CD226 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9298-DN

R&D Systems, part of Bio-Techne
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Key Product Details

Source

HEK293

Accession #

Q15762

Conjugate

Unconjugated

Applications

Bioactivity

View all DNAM-1/CD226 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human DNAM-1/CD226 protein
Glu19-Asn247, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu19

Predicted Molecular Mass

27 kDa

SDS-PAGE

31-55 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human DNAM-1/CD226 is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human Nectin-2/CD112 Fc Chimera (Catalog # 9317-N2) binds with an ED50 of 0.4-2 μg/mL.

Scientific Data Images for Recombinant Human DNAM-1/CD226 His-tag Protein, CF

Recombinant Human DNAM-1/CD226 His-tag Protein Bioactivity

Recombinant Human DNAM-1/CD226 His-tag Protein Bioactivity

When Recombinant Human DNAM-1/CD226 (Catalog # 9298-ND) is coated at 0.5 µg/mL, 100 µL/well, Recombinant Nectin-2/ CD112 Fc Chimera (Catalog # 9317-N2) binds with an ED50 of 0.4-2 µg/mL.

Formulation, Preparation and Storage

9298-DN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DNAM-1/CD226

DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily (1). Mature human DNAM-1 contains a 236 amino acid (aa) extracellular domain (ECD) with two Ig-like C2-set domains and a 61 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins (1, 2). Within the ECD, human DNAM-1 shares 50% and 52% aa sequence identity with mouse and rat DNAM-1, respectively. DNAM-1 is expressed on multiple lymphoid and myeloid cells and interacts with CD155/PVR and Nectin-2/CD112 (3, 4). Ligation of DNAM-1 promotes the activation of NK cells, CD8+ T cells, and mast cells (2-6), dendritic cell maturation, megakaryocyte and activated platelet adhesion to vascular endothelial cells, and monocyte extravasation; it inhibits the formation of osteoclasts (7-10). Platelet-endothelium interactions mediated by DNAM-1 can enable the metastasis of tumor cells to the lung (11). CD96 competes with DNAM-1 for binding to CD155 and blocks DNAM-1 mediated NK cell activation (12). In activated, but not in resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to the tyrosine and serine phosphorylation of DNAM-1 during activation (6, 9, 13-15).

References

  1. Zingoni, A. et al. (2013) Front. Immunol. 3:408.
  2. Shibuya, A. et al. (1996) Immunity 4:573.
  3. Bottino, C. et al. (2003) J. Exp. Med. 198:557.
  4. Tahara-Hanaoka, S. et al. (2004) Int. Immunol. 16:533.
  5. Dardalhon, V. et al. (2005) J. Immunol. 175:1558.
  6. Bachelet, I. et al. (2006) J. Biol. Chem. 281:27190.
  7. Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
  8. Kakehi, S. et al. (2007) Mol. Cell. Biochem. 301:209.
  9. Kojima, H. et al. (2003) J. Biol. Chem. 278:36748.
  10. Tahara-Hanaoka, S. et al. (2006) Blood 107:1491.
  11. Morimoto, K. et al. (2007) Oncogene 27:264.
  12. Chan, C.J. et al. (2014) Nat. Immunol. 15:431.
  13. Shibuya, K. et al. (1999) Immunity 11:615.
  14. Shibuya, K. et al. (2003) J. Exp. Med. 198:1829.
  15. Shibuya, A. et al. (1998) J. Immunol. 166:1671.

Long Name

DNAX Accessory Molecule 1

Alternate Names

CD226, DNAM1, PTA1, TLiSA1

Entrez Gene IDs

10666 (Human); 225825 (Mouse); 307199 (Rat); 102117316 (Cynomolgus Monkey)

Gene Symbol

CD226

UniProt

Q15762

Additional DNAM-1/CD226 Products

Product Documents for Recombinant Human DNAM-1/CD226 His-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human DNAM-1/CD226 His-tag Protein, CF

For research use only

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