Recombinant Human Nectin-2/CD112 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9317-N2

R&D Systems, part of Bio-Techne
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9317-N2-050
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Key Product Details

Source

HEK293

Accession #

NP_002847.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Nectin-2/CD112 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Nectin-2/CD112 protein
Human Nectin-2/CD112
(Gln32-Leu360)
Accession # NP_002847.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE with silver staining, under reducing conditions.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln32 predicted

Predicted Molecular Mass

62 kDa

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Nectin‑2/CD112 Fc Chimera is immobilized at 0.5 µg/mL (100 µL/well), the concentration of Recombinant Human DNAM‑1 Fc Chimera (Catalog # 666-DN) that produces 50% of the optimal binding response is 0.04-0.2 μg/mL.

Scientific Data Images for Recombinant Human Nectin-2/CD112 Fc Chimera Protein, CF

Recombinant Human Nectin-2/CD112 Fc Chimera Protein Bioactivity

Recombinant Human Nectin-2/CD112 Fc Chimera Protein Bioactivity

When Recombinant Human Nectin-2/CD112 Fc Chimera (Catalog # 9317-N2) is coated at 0.5 µg/mL, Recombinant Human DNAM-1 Fc Chimera (Catalog # 666-DN) binds with an ED50 of 0.04-0.2 µg/mL.

Formulation, Preparation and Storage

9317-N2
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nectin-2/CD112

Nectins are a small family of Ca++-independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that organize intercellular junctions (1). They are highly homologous to the human receptor for poliovirus, and as such have been alternately named poliovirus receptor-related proteins. The 65 kDa long isoform of human Nectin-2/CD112 (Nectin-2 delta) consists of a 329 amino acid (aa) extracellular region (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 157 aa cytoplasmic domain (2). Within the ECD, human Nectin-2 shares 72% aa sequence identity with mouse Nectin-2. Alternative splicing generates a short 60 kDa isoform with a 94 aa cytoplasmic tail (2). Nectin-2 localizes to adherens junctions between neurons, endothelial cells, epithelial cells, and fibroblasts (1, 3). It forms homodimers in cis, followed by dimers in trans (between cells) (3). It does not cis-dimerize with other Nectins but forms cis-dimers between its two splice forms. Notably, a Nectin-2 cis-dimer on one cell can heterodimerize with a Nectin-3 cis-dimer on a neighboring cell (3). Nectin-2 additionally binds to DNAM-1/CD226 on NK cells and triggers NK cell cytolytic activity (4, 5). Nectin-2 is known to bind pseudorabies virus and herpes simplex virus-2 (HSV-2), but not HSV-1 or poliovirus (3, 6). Nectin-2 is a component of cardiac intercalated discs and limits fibrosis and dysfunction resulting from pressure overload (7).

References

  1. Samanta, D. and S.C. Almo (2015) Cell. Mol. Life Sci. 72:645.
  2. Eberle, F. et al. (1995) Gene 159:267.
  3. Struyf, F. et al. (2002) J. Virol. 76:12940.
  4. Bottino, C. et al. (2003) J. Exp. Med. 198:557.
  5. Pende, D. et al. (2005) Mol. Immunol. 42:463.
  6. Warner, M.S. et al. (1998) Virology 246:179.
  7. Satomi-Kobayashi, S. et al. (2009) Hypertension 54:825.

Long Name

Poliovirus Receptor Related 2

Alternate Names

CD112, HVEB, MPH, Nectin2, PRR2, PVRL2

Entrez Gene IDs

5819 (Human); 19294 (Mouse); 102137456 (Cynomolgus Monkey)

Gene Symbol

NECTIN2

UniProt

NP_002847.1 (Human)

Additional Nectin-2/CD112 Products

Product Documents for Recombinant Human Nectin-2/CD112 Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human Nectin-2/CD112 Fc Chimera Protein, CF

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