Recombinant Human Dopamine beta-Hydroxylase Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7376-AO

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
7376-AO-010
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

CHO

Accession #

P09172

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Dopamine beta-Hydroxylase Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Dopamine beta-Hydroxylase protein
Met1-Gly617, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu37 & Ser40

Predicted Molecular Mass

66 kDa

SDS-PAGE

65-85 kDa, reducing conditions

Activity

Measured by its ability to convert tyramine to octopamine.
The specific activity is >7,000 pmol/min/μg, as measured under the described conditions.

Reviewed Applications

Read 1 review rated 4 using 7376-AO in the following applications:

Formulation, Preparation and Storage

7376-AO
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Dopamine beta-Hydroxylase

Dopamine beta-Hydroxylase (DBH), also known as dopamine beta-monooxygenase, belongs to the copper type II, ascorbate-dependent monooxygenase family. DBH is found within the neurosecretory vesicles of adrenal medullae and the large dense‑cored synaptic vesicles of the sympathetic nervous system as both membrane‑associated and soluble forms (1, 2). It catalyzes the conversion of dopamine to noradrenaline in sympathetic neurons, making it an important enzyme for catecholamine biosynthesis (3, 4). Mutations in the DBH gene that result in low DBH activity are a cause of noradrenaline deficiency (5). This recombinant human DBH is the secreted, soluble form.

References

  1. Dhawan, S. et al. (1987) J. Biol. Chem. 262:1869.
  2. Saxena, A. and P.J. Fleming (1983) J. Biol. Chem. 258:4147.
  3. Levin, E.Y. et al. (1960) J. Biol. Chem. 235:2080.
  4. Friedman, S. and S. Kaufman (1965) J. Biol. Chem. 240:4763.
  5. Kim, C.H. et al. (2002) Am. J. Med. Genet. 108:140.

Alternate Names

DBH, DBM, Dopamine betaHydroxylase, DOPBHY

Entrez Gene IDs

1621 (Human); 13166 (Mouse); 25699 (Rat)

Gene Symbol

DBH

UniProt

P09172

Additional Dopamine beta-Hydroxylase Products

Product Documents for Recombinant Human Dopamine beta-Hydroxylase Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human Dopamine beta-Hydroxylase Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.