Recombinant Human DPPII/QPP/DPP7 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 3438-SE
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human DPPII/QPP/DPP7 protein
Gly22-Leu492, with a C-terminal 10-His tag
Gly22-Leu492, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Gly22 & Arg24
Predicted Molecular Mass
54 kDa
SDS-PAGE
64 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Lys-Pro-AMC (KP-AMC).
The specific activity is >4,000 pmol/min/µg, as measured under the described conditions.
The specific activity is >4,000 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
3438-SE
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: DPPII/QPP/DPP7
Dipeptidyl-peptidase II (DPPII) is identical to quiescent cell proline dipeptidase (QPP) and dipeptidylpeptidase 7 (DPP7) (1, 2). It shares some substrate and cleavage specificity with DPPIV/CD26, DPP8, DPP9 and seprase/FAP (fibroblast activation protein), members of the S09 family of serine proteases. As prolyl proteases that cleave proteins and peptides after proline residues, these enzymes have high potential for drug discovery (3, 4). However, DPP7 is not a member of the S09 family, but a member of the S28 family that also includes lysosomal Pro‑X carboxypeptidase/prolylcarboxypeptidase/PRCP and thymus-specific serine peptidase/PRSS16 (2). The human DPP7 precursor consists of a signal peptide (aa 1‑21) and a mature chain (aa 22‑492). The purified rhDPP7 is active against Lys‑Pro-AMC and Lys‑Ala-AMC. Its activity against Lys‑Pro‑AMC is approximately 10-fold of that against Lys‑Ala‑AMC under otherwise identical conditions.
References
- Underwood, R. et al. (1999) J. Biol. Chem. 274:34053.
- Maes, M.B. et al. (2005) Biochem. J. 386:315.
- Rosenblum, J.S. and J.W. Kozarich (2003) Curr. Opin. Chem. Biol. 7:496.
- Lankas, G.R. et al. (2005) Diabetes 54:2988.
Long Name
Dipeptidyl-peptidase II
Alternate Names
DPP7, QPP
Gene Symbol
DPP7
UniProt
Additional DPPII/QPP/DPP7 Products
Product Documents for Recombinant Human DPPII/QPP/DPP7 Protein, CF
Product Specific Notices for Recombinant Human DPPII/QPP/DPP7 Protein, CF
For research use only
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