Recombinant Human EBI3 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 11084-EI
Key Product Details
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human EBI3 protein
Arg21-Lys220
Arg21-Lys220
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Arg21
Predicted Molecular Mass
23 kDa
SDS-PAGE
30-40 kDa, under reducing conditions.
Activity
Measured by its binding ability in a functional ELISA.
When Recombinant Human EBI3 Protein (Catalog # 11084-EI) is immobilized at 5.0 μg/mL (100 μL/well), the concentration of Recombinant Mouse IL-27 p28/IL-30 Protein (Catalog # 7430-ML) that produces 50% of the optimal binding response is 40.0-300 ng/mL.
When Recombinant Human EBI3 Protein (Catalog # 11084-EI) is immobilized at 5.0 μg/mL (100 μL/well), the concentration of Recombinant Mouse IL-27 p28/IL-30 Protein (Catalog # 7430-ML) that produces 50% of the optimal binding response is 40.0-300 ng/mL.
Scientific Data Images for Recombinant Human EBI3 Protein, CF
Recombinant Human EBI3 Protein Binding Activity.
Measured by its binding ability in a functional ELISA. When Recombinant Human EBI3 Protein (Catalog # 11084-EI) is immobilized at 5.0 μg/mL (100 μL/well), the concentration of Recombinant Mouse IL-27 p28/IL-30 Protein (7430-ML) that produces 50% of the optimal binding response is 40.0-300 ng/mL.
Recombinant Human EBI3 Protein SDS-PAGE.
2 μg/lane of Recombinant Human EBI3 Protein (Catalog # 11084-EI) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 30-40 kDa.Formulation, Preparation and Storage
11084-EI
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 200 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: EBI3
References
- Devergne, O. et al. (1996) J. Virol. 70:1143.
- Devergne, O. et al. (1997) Protc. Natl. Acad. Sci. U.S.A. 94:12041.
- Pflanz, S. et al. (2002) Immunity 16:779.
- Wang, X. et al. (2016) Eur. J. Immuno. 46:1343.
- Cassatella, M.A. et al. (2020) J Leukoc. Biol. 108:1515.
- Chehboun, S. et al. (2017) J Biol. Chem. 292:6644.
Long Name
Epstein-Barr virus induced gene 3
Alternate Names
EBI-3, IL-27 beta, IL-27B, IL-35 EBI3, IL35 EBI3
Gene Symbol
EBI3
UniProt
Additional EBI3 Products
Product Documents for Recombinant Human EBI3 Protein, CF
Product Specific Notices for Recombinant Human EBI3 Protein, CF
For research use only
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