Recombinant Human EGFR Isoform vIII Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9565-ER

R&D Systems, part of Bio-Techne
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9565-ER-050
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Key Product Details

Source

NS0

Accession #

NP_001333870

Conjugate

Unconjugated

View all EGFR Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human EGFR protein
Leu25-Ser378 (Asn273Lys), with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu25

Predicted Molecular Mass

39 kDa

SDS-PAGE

65 - 80 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

9565-ER
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGFR

The epidermal growth factor receptor (EGFR) subfamily of receptor tyrosine kinases comprises four members: EGFR (also known as HER1, ErbB1 or ErbB), ErbB2 (Neu, HER2), ErbB3 (HER3), and ErbB4 (HER4). All family members are type I transmembrane glycoproteins that have an extracellular domain which contains two cysteine-rich domains separated by a spacer region that is involved in ligand binding, and a cytoplasmic domain which has a membrane-proximal tyrosine kinase domain and a C-terminal tail with multiple tyrosine autophosphorylation sites. The human EGFR gene encodes a 1210 amino acid (aa) residue precursor with a 24 aa putative signal peptide, a 621 aa extracellular domain, a 23 aa transmembrane domain, and a 542 aa cytoplasmic domain (1-3). Human EGFR shares 90% identity with mouse and rat EGFR. The amino acid 25-378 of EGFR encodes the most common extracellular domain truncation mutant, termed EGFRvIII. It has been proved that EGFRvIII is relevant in a number of cancer types. EGFRvIII is tumor specific, and has not been found in normal tissues. EGFRvIII is highly tumorigenic, and can cause aggressive tumor phenotype, with increased invasion, proliferation, angiogenesis, and evasion of apoptosis. This EGFRvIII-mediated enhanced tumourigenicity combined with the lack of EGFRvIII expression in normal tissue makes it an ideal therapeutic targets (4).

References

  1. Daly, R.J. (1999) Growth Factors, 16:255.
  2. Schlessinger, J. (2000) Cell. 103:211.
  3. Maihle, N.J. et al. (2002) Cancer Treat. Res. 107:247.
  4. Kuan, C. T. et al. (2001) Endocrine-related cancer 8:83.

Long Name

Epidermal Growth Factor Receptor

Alternate Names

EGF R, ErbB, ErbB1, HER-1

Entrez Gene IDs

1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)

Gene Symbol

EGFR

UniProt

NP_001333870

Additional EGFR Products

Product Documents for Recombinant Human EGFR Isoform vIII Protein, CF

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Product Specific Notices for Recombinant Human EGFR Isoform vIII Protein, CF

For research use only

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