Recombinant Human EGFR Kinase Domain His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11142-ER

R&D Systems, part of Bio-Techne
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11142-ER-020
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Key Product Details

Source

Sf 21 (baculovirus)

Accession #

P00533.2

Conjugate

Unconjugated

Applications

Enzyme Activity

View all EGFR Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human EGFR protein
Gly696-Gly1022, with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

38 kDa

SDS-PAGE

39 kDa, under reducing conditions

Activity

Measured by its ability to transfer phosphate from adenosine triphosphate (ATP) to a synthetic peptide substrate.
The specific activity is >35 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant Human EGFR Kinase Domain His-tag Protein, CF

Recombinant Human EGFR Kinase Domain His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human EGFR Kinase Domain His-tag Protein (Catalog # 11142-ER) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 39 kDa.

Formulation, Preparation and Storage

11142-ER
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and DTT.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: EGFR

The EGFR subfamily of receptor tyrosine kinases comprises four members: EGFR (also known as HER-1, ErbB1, or ErbB), ErbB2 (Neu, HER-2), ErbB3 (HER-3), and ErbB4 (HER-4). All family members are type I transmembrane glycoproteins with an extracellular ligand binding domain containing two cysteine-rich domains separated by a spacer region and a cytoplasmic domain containing a membrane-proximal tyrosine kinase domain followed by multiple tyrosine autophosphorylation sites (1,2). The human EGFR cDNA encodes a 1210 amino acid (aa) precursor with a 24 aa signal peptide, a 621 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 542 aa cytoplasmic domain (3,4). The kinase domain contains the conserved catalytic domain with an ATP binding site in the cleft of two lobes at the N-terminal and C-terminal regions and an activation loop with a DFG motif that changes conformation to regulate activity (5-7). EGFR binds a subset of the EGF family ligands (1,2) that induce EGFR homodimerization as well as heterodimerization with other family members resulting in kinase activation and cell signaling (8-10). EGFR signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis (11,12). EGFR has been reported as overexpressed and/or mutated in tumors with high levels of activation of EGFR related to poor prognosis and tumor regression rates (13,14). EGFR kinase inhibition is a promising target for cancer therapeutics and includes development of drugs to address EGFR-mutation conferred resistance (7,15).

References

  1. Singh, A.B. and R.C. Harris (2005) Cell. Signal. 17:1183.
  2. Shilo, B.Z. (2005) Development 132:4017.
  3. Lin, C. et al. (1984) Science 224:843.
  4. Ullrich, A. et al. (1984) Nature 309:418.
  5. Stamos, J. et. al. (2002) J. Biol. Chem. 277:46265.
  6. Liu, Q. et al. (2013) Chem. Biol. 20:146.
  7. Amelia, T. et al. (2022) Molecules 27:819.
  8. Graus-Porta, D. et al. (1997) EMBO J. 16:1647.
  9. Yarden, Y. et al. (2001) Nat. Rev. Mol. Cell Biol. 2:127. 
  10. Lemmon, M.A. (2009) Exp. Cell Res. 315:638.
  11. Sibilia, M. and E.F. Wagner (1995) Science 269:234.
  12. Miettinen, P.J. et al. (1995) Nature 376:337.
  13. Janne, P.A. et al. (2005) J. Clin. Oncol. 23:3227.
  14. Yun, C.-H. et al. (2008) Proc. Nat. Acad. Sci. USA 105:2070.
  15. Yu, J. et al. (2022) Front. Mol. Biosci. 9:847825.

Long Name

Epidermal Growth Factor Receptor

Alternate Names

EGF R, ErbB, ErbB1, HER-1

Entrez Gene IDs

1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)

Gene Symbol

EGFR

UniProt

P00533.2

Additional EGFR Products

Product Documents for Recombinant Human EGFR Kinase Domain His-tag Protein, CF

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Product Specific Notices for Recombinant Human EGFR Kinase Domain His-tag Protein, CF

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