Recombinant Human Endorepellin Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 2364-ER

R&D Systems, part of Bio-Techne
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2364-ER-050
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Key Product Details

Source

NS0

Accession #

P98160

Conjugate

Unconjugated

Applications

Bioactivity

View all Endorepellin/Perlecan Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Endorepellin/Perlecan protein
Arg3684-Ser4391 (Ser4004Thr), with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg3684

Predicted Molecular Mass

76.7 kDa

SDS-PAGE

90-95 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human VEGF R1/Flt-1 (Catalog # 321-FL) is coated at 5 μg/mL (100 μL/well), the concentration of Recombinant Human Endorepellin/Perlecan that produces 50% of the optimal binding response is approximately 0.75-6 μg/mL.

Reviewed Applications

Read 2 reviews rated 5 using 2364-ER in the following applications:

Scientific Data Images for Recombinant Human Endorepellin Protein, CF

Recombinant Human Endorepellin Protein Binding Activity

Recombinant Human Endorepellin Protein Binding Activity

When Recombinant Human VEGFR1/Flt-1 Fc Chimera (Catalog # 321-FL) is immobilized at 5 µg/mL (100 µL/well), Recombinant Human Endorepellin/Perlecan (Catalog # 2364-ER) binds with an ED50 of 0.75-6 µg/mL.

Formulation, Preparation and Storage

2364-ER
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endorepellin/Perlecan

Endorepellin is an 80 kDa glycoprotein that is derived from the C-terminal end of the heparan sulfate proteoglycan, Perlecan. Perlecan itself is an approximately 850 kDa modular protein that contains multiple LDLR, EGF-like, Laminin-like, and immunoglobulin-like domains. Mouse Perlecan lacks several of the Ig-like domains found in the human protein (1-3). Endorepellin corresponds to domain V of Perlecan and consists of three Laminin G domains separated by four EGF-like domains (4). Human Endorepellin shares 89% amino acid sequence identity with mouse Endorepellin. A 26 kDa fragment of Endorepellin is known as LG3 and contains the third Laminin G-like domain of Endorepellin. LG3 can be released by Cathepsin L or BMP-1/Tolloid family mediated cleavage (4-6). Endorepellin binds to Integrin alpha2/ beta1, preventing the integrin-dependent adhesion of vascular endothelial cells (EC) to fibronectin and collagen I (7, 8). In contrast, this interaction enhances platelet adhesion to collagen as well as platelet aggregation and activation (9). Endorepellin additionally binds to VEGF R1 and VEGF R2 on EC (10). Its binding to VEGF R2 and Integrin alpha2/ beta1 on EC induces the association and down‑regulation of both proteins, followed by the inhibition of EC migration, tubulogenesis, secretion of VEGF, and the activation of multiple receptors involved in angiogenesis (4, 7, 8, 10, 11). Endorepellin also binds to Endostatin, resulting in a reduction of the anti-angiogenic activity of both proteins (4). It preferentially interacts with tumor vasculature and inhibits tumor growth and angiogenesis (8, 12).

References

  1. Whitelock, J.M. et al. (2008) Biochemistry 47:11174.
  2. Murdoch, A.D. et al. (1992) J. Biol. Chem. 267:8544.
  3. Noonan, D.M. et al. (1991) J. Biol. Chem. 266:22939.
  4. Mongiat, M. et al. (2003) J. Biol. Chem. 278:4238.
  5. Cailhier, J.-F. et al. (2008) J. Biol. Chem. 283:27220.
  6. Gonzalez, E.M. et al. (2005) J. Biol. Chem. 280:7080.
  7. Bix, G. et al. (2004) J. Cell Biol. 166:97.
  8. Woodall, B.P. et al. (2008) J. Biol. Chem. 283:2335.
  9. Bix, G. et al. (2007) Blood 109:3745.
  10. Goyal, A. et al. (2011) J. Biol. Chem. 286:25947.
  11. Nystrom, A. et al. (2009) Blood 114:4897.
  12. Bix, G. et al. (2006) J. Natl. Cancer Inst. 98:1634.

Long Name

Heparan Sulfate Proteoglycan

Alternate Names

HSPG2, Perlecan

Entrez Gene IDs

3339 (Human); 15530 (Mouse)

Gene Symbol

HSPG2

UniProt

P98160

Additional Endorepellin/Perlecan Products

Product Documents for Recombinant Human Endorepellin Protein, CF

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Product Specific Notices for Recombinant Human Endorepellin Protein, CF

For research use only

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